Spatial distribution of microbial communities in the cystic fibrosis lung

Willner, Dana, Haynes, Matthew R., Furlan, Mike, Schmieder, Robert, Lim, Yan Wei, Rainey, Paul B., Rohwer, Forest and Conrad, Douglas (2012) Spatial distribution of microbial communities in the cystic fibrosis lung. ISME Journal, 6 2: 471-474. doi:10.1038/ismej.2011.104


Author Willner, Dana
Haynes, Matthew R.
Furlan, Mike
Schmieder, Robert
Lim, Yan Wei
Rainey, Paul B.
Rohwer, Forest
Conrad, Douglas
Title Spatial distribution of microbial communities in the cystic fibrosis lung
Journal name ISME Journal   Check publisher's open access policy
ISSN 1751-7362
1751-7370
Publication date 2012-02
Year available 2011
Sub-type Article (original research)
DOI 10.1038/ismej.2011.104
Volume 6
Issue 2
Start page 471
End page 474
Total pages 4
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2012
Language eng
Abstract Cystic fibrosis (CF) is a common fatal genetic disorder with mortality most often resulting from microbial infections of the lungs. Culture-independent studies of CF-associated microbial communities have indicated that microbial diversity in the CF airways is much higher than suggested by culturing alone. However, these studies have relied on indirect methods to sample the CF lung such as expectorated sputum and bronchoalveolar lavage (BAL). Here, we characterize the diversity of microbial communities in tissue sections from anatomically distinct regions of the CF lung using barcoded 16S amplicon pyrosequencing. Microbial communities differed significantly between different areas of the lungs, and few taxa were common to microbial communities in all anatomical regions surveyed. Our results indicate that CF lung infections are not only polymicrobial, but also spatially heterogeneous suggesting that treatment regimes tailored to dominant populations in sputum or BAL samples may be ineffective against infections in some areas of the lung.
Keyword Cystic fibrosis
Amplicon sequencing
Microbial communities
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes "Short communication". Published online 28 July 2011.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Chemistry and Molecular Biosciences
UQ Diamantina Institute Publications
 
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Created: Thu, 20 Oct 2011, 08:54:39 EST by Dana Willner on behalf of School of Chemistry & Molecular Biosciences