Case studies of the spatial heterogeneity of DNA viruses in the cystic fibrosis lung

Willner, Dana, Haynes, Matthew R., Furlan, Mike, Hanson, Nicole, Kirby, Breeann, Lim, Yan Wei, Rainey, Paul B., Schmieder, Robert, Youle, Merry, Conrad, Douglas and Rohwer, Forest (2012) Case studies of the spatial heterogeneity of DNA viruses in the cystic fibrosis lung. American Journal of Respiratory Cell and Molecular Biology, 46 2: 127-131. doi:10.1165/rcmb.2011-0253OC

Author Willner, Dana
Haynes, Matthew R.
Furlan, Mike
Hanson, Nicole
Kirby, Breeann
Lim, Yan Wei
Rainey, Paul B.
Schmieder, Robert
Youle, Merry
Conrad, Douglas
Rohwer, Forest
Title Case studies of the spatial heterogeneity of DNA viruses in the cystic fibrosis lung
Journal name American Journal of Respiratory Cell and Molecular Biology   Check publisher's open access policy
ISSN 1044-1549
Publication date 2012-02-01
Year available 2011
Sub-type Article (original research)
DOI 10.1165/rcmb.2011-0253OC
Volume 46
Issue 2
Start page 127
End page 131
Total pages 5
Place of publication New York, United States
Publisher American Thoracic Society
Collection year 2013
Language eng
Abstract Microbial communities in the lungs of cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD) patients have been shown to be spatially heterogeneous. Viral communities may also vary spatially, leading to localized viral populations and infections. Here, we characterized viral communities from multiple areas of the lungs of two late-stage CF patients using metagenomics: the explanted lungs from transplant patient and lungs acquired post-mortem. All regions harbored eukaryotic viruses which may infect the human host, notably herpesviruses, anelloviruses, and papillomaviruses. In the highly diseased apical lobes of the explant lungs, viral diversity was extremely low and only eukaryotic viruses were present. The absence of phage suggests that CF-associated microbial biofilms may escape top-down controls by phage predation. The phage present in other lobes of the explant lungs and in all lobes of the post-mortem lungs comprised distinct communities, and encoded genes for clinically important microbial phenotypes, including small colony variants and antibiotic resistance. Based on the these observations, we postulate that viral communities in CF lungs are spatially distinct and contribute to CF pathology by augmenting the metabolic potential of the resident microbes as well as by directly damaging lung tissue via carcinomas and herpesviral outbreaks.
Keyword Cystic fibrosis
Antibiotic resistance
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published ahead of print on October 6, 2011

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Chemistry and Molecular Biosciences
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Created: Thu, 20 Oct 2011, 08:49:18 EST by Dana Willner on behalf of School of Chemistry & Molecular Biosciences