The Thlaspi caerulescens NRAMP homologue TcNRAMP3 is capable of divalent cation transport

Wei, Wei, Chai, Tuanyao, Zhang, Yuxiu, Han, Lu, Jin, Xu and Guan, Ziqiu (2009) The Thlaspi caerulescens NRAMP homologue TcNRAMP3 is capable of divalent cation transport. Molecular Biotechnology, 41 1: 15-21. doi:10.1007/s12033-008-9088-x


Author Wei, Wei
Chai, Tuanyao
Zhang, Yuxiu
Han, Lu
Jin, Xu
Guan, Ziqiu
Title The Thlaspi caerulescens NRAMP homologue TcNRAMP3 is capable of divalent cation transport
Formatted title
The Thlaspi caerulescens NRAMP homologue TcNRAMP3 is capable of divalent cation transport
Journal name Molecular Biotechnology   Check publisher's open access policy
ISSN 1073-6085
1559-0305
Publication date 2009-01
Year available 2008
Sub-type Article (original research)
DOI 10.1007/s12033-008-9088-x
Volume 41
Issue 1
Start page 15
End page 21
Total pages 7
Place of publication Totowa, NJ, United States
Publisher Humana Press
Language eng
Formatted abstract
The NRAMP gene family encodes integral membrane protein and mediates the transport of Fe, however, its function in transport of toxic metal ions is not very clear in plants. TcNRAMP3 was isolated from Thlaspi caerulescens, and encoded a metal transporter member of the NRAMP family. TcNRAMP3 was predominantly expressed in roots of T. caerulescens by semi-quantitative RT-PCR. The expression of TcNRAMP3 was induced by iron starvation and by the heavy metals Cd and Ni in roots. TcNRAMP3 was able to rescue growth of an iron uptake fet3fet4 mutant yeast strain, suggesting a possible role in iron transport. Expression of TcNRAMP3 in yeast increased Cd sensitivity and Cd content, while it enhanced the Ni resistance and reduced Ni accumulation, indicating that TcNRAMP3 could accumulate Cd and exclude Ni in yeast. Furthermore, overexpression of TcNRAMP3 in tobacco resulted in slight Cd sensitivity of root growth and did not influence Ni resistance. These results suggested that TcNRAMP3 played a role in metal cation homeostasis in plant.
Keyword Heavy metal
Metal transporter
NRAMP
Thlaspi caerulescens
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Published online: 29 July 2008

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
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Created: Wed, 19 Oct 2011, 13:51:30 EST by Wei Wei on behalf of Queensland Brain Institute