A novel and practical method using HRP-Polymer Conjugate and Microwave treatment for visualisation of 2 antigens raised from the same or different species in paraffin-embedded tissues

Chan, Anthony, Matias, Marie Anne Teresa and Farah, Camile Selim (2011) A novel and practical method using HRP-Polymer Conjugate and Microwave treatment for visualisation of 2 antigens raised from the same or different species in paraffin-embedded tissues. Applied Immunohistochemistry & Molecular Morphology, 19 4: 376-383. doi:10.1097/PAI.0b013e31820251c0


Author Chan, Anthony
Matias, Marie Anne Teresa
Farah, Camile Selim
Title A novel and practical method using HRP-Polymer Conjugate and Microwave treatment for visualisation of 2 antigens raised from the same or different species in paraffin-embedded tissues
Journal name Applied Immunohistochemistry & Molecular Morphology
ISSN 1541-2016
Publication date 2011-07
Sub-type Article (original research)
DOI 10.1097/PAI.0b013e31820251c0
Volume 19
Issue 4
Start page 376
End page 383
Total pages 8
Place of publication United States
Publisher Lippincott Williams & Wilkins
Collection year 2012
Language eng
Abstract Recent advances in imaging systems have improved the ability to simultaneously visualize expression of multiple antigens in a single tissue section. In most instances, fluorescence microscopy, modern imaging systems, electronic scanners, and expensive reagents are required to achieve these results. Using conventional methodologies, we have developed an inexpensive and straightforward method for visualizing 2 antigens on a single formalin-fixed paraffin-embedded tissue section. The protocol combines the use of broad spectrum horseradish peroxidase-polymer conjugate and microwave treatment for detection of 2 primary antibodies raised from the same or different species. Our technique enables the researcher to simultaneously visualize 2 antigens in paraffin-embedded tissue requiring only one enzyme and a microwave.
Keyword Double staining
HPR-polymer conjugate
Immunohistochemistry
Microwave
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Dentistry Publications
 
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Created: Wed, 19 Oct 2011, 13:32:04 EST by Ms Marie Matias on behalf of School of Dentistry