Validation of a commercial enzyme immunoassay for detection of Clostridium difficile toxins in feces of horses with acute diarrhea

Medina-Torres, C. E., Weese, J. S. and Staempfli, H. R. (2010) Validation of a commercial enzyme immunoassay for detection of Clostridium difficile toxins in feces of horses with acute diarrhea. Journal of Veterinary Internal Medicine, 24 3: 628-632. doi:10.1111/j.1939-1676.2010.00506.x


Author Medina-Torres, C. E.
Weese, J. S.
Staempfli, H. R.
Title Validation of a commercial enzyme immunoassay for detection of Clostridium difficile toxins in feces of horses with acute diarrhea
Journal name Journal of Veterinary Internal Medicine   Check publisher's open access policy
ISSN 0891-6640
1939-1676
Publication date 2010-05
Sub-type Article (original research)
DOI 10.1111/j.1939-1676.2010.00506.x
Open Access Status DOI
Volume 24
Issue 3
Start page 628
End page 632
Total pages 5
Place of publication Hoboken, NJ, U.S.A.
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
Background: Clostridium difficile infection (CDI) is a recognized cause of colitis in the horse. Identification of its toxins is important for management of individual cases and for prevention of transmission and zoonosis. In humans, CDI diagnosis is performed with enzyme immunoassays, none of which have been validated for horses.

Hypothesis/Objectives:
(1) Establish which test for CDI diagnosis was more frequently used by diagnostic laboratories, (2) determine the identified test’s performance, sensitivity, and specificity, and (3) validate its use in diarrheic horses.

Animals:
Samples were obtained from 72 horses presented with acute diarrhea and hospitalized at the Ontario Veterinary College, University of Guelph.

Methods: A survey was conducted to establish which of the tests for CDI diagnosis in horses is most commonly used throughout North America. A questionnaire was sent to all laboratories registered in the Veterinary Infection Control Society and the American Association of Veterinary Laboratory Diagnosticians. The performance of the test was evaluated by comparison to a cell cytotoxicity assay (CTA), the accepted Gold Standard for C. difficile toxin detection.

Results: The Techlab C. difficile Tox A/B II ELISA was the most frequently used test. Compared with the CTA, no significant difference was observed, and a good level of agreement (93%) was obtained. The diagnostic performance of the ELISA test was adequate (84% sensitivity and 96% specificity).

Conclusions and Clinical Importance:
Results demonstrate that the Techlab C. difficile Tox A/B II ELISA is a reliable, adequate, and practical tool for identification of C. difficile toxins in horse feces.
Keyword Colitis
Diagnosis
ELISA
Equine
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Published May-June 2010.

Document type: Journal Article
Sub-type: Article (original research)
Collections: ERA 2012 Admin Only
School of Veterinary Science Publications
 
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Created: Wed, 19 Oct 2011, 12:30:24 EST by Carlos Medina-torres on behalf of School of Veterinary Science