Human endogenous retrovirus K10: expression of Gag protein and detection of antibodies in patients with seminomas

Sauter, Marlies, Schommer, Stefanie, Kremmer, Elisabeth, Remberger, Klaus, Doelken, Gottfried, Lemm, Ina, Buck, Marion, Best, Barbara, Neumann-Haefelin, Dieter and Mueller-Lantzsch, Nikolaus (1995) Human endogenous retrovirus K10: expression of Gag protein and detection of antibodies in patients with seminomas. Journal of Virology, 69 1: 414-421.

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Author Sauter, Marlies
Schommer, Stefanie
Kremmer, Elisabeth
Remberger, Klaus
Doelken, Gottfried
Lemm, Ina
Buck, Marion
Best, Barbara
Neumann-Haefelin, Dieter
Mueller-Lantzsch, Nikolaus
Title Human endogenous retrovirus K10: expression of Gag protein and detection of antibodies in patients with seminomas
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
Publication date 1995-01
Year available 1995
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 69
Issue 1
Start page 414
End page 421
Total pages 8
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract The human endogenous retrovirus K10 (HERV-K10) has been identified in the human genome by its homology to retroviruses of other vertebrates (M. Ono, T. Yasunaga, T. Miyata, and H. Ushikubo, J. Virol. 60:589-598, 1986). Using PCR amplification, DNA cloning, sequencing, and procaryotic expression, we were able to demonstrate that HERV-K10 encodes a 73-kDa protein which was processed by a HERV-K10-encoded protease to yield proteins p22/p26, p30, and p15/16. Analysis of the teratocarcinoma cell line Tera 1 or tumor tissues by immunoblotting demonstrated that the 80-kDa polyprotein of HERV-K10 gag and a processed protein of 39 kDa were expressed. In addition, a major protein of 39 kDa and additional species of 30, 22, 19, and 17 kDa could be detected in the supernatant of Tera 1 cells, suggesting that HERV-K10 Gag proteins are either secreted or processed to probably incomplete viral particles. In addition, the gag gene of HERV-K10 was expressed in the baculovirus system. Using this recombinant system to test antisera from patients with different diseases and healthy individuals, we were able to detect antibodies against the N-terminal part of HERV-K10 Gag in 2 to 4% of groups of tumor patients with titers ranging between 1:80 and 1:640, while approximately 0.1 to 0.5% of healthy individuals exhibited antibodies with lower titers. In contrast, patients with seminoma had antibody titers in the range of 1:2,560 at the time when the tumor was detected. Immunohistochemistry using specific rabbit sera or monoclonal antibodies against HERV-K10 Gag revealed that the Gag protein is expressed in the cytoplasm of the tumor cells. Furthermore, an 80-kDa protein corresponding to the HERV-K10 Gag polyprotein could be detected in tumor biopsies. For the first time, these data indicate that HERV-K10 Gag proteins are synthesized in seminoma cells and tumors exhibit relatively high antibody titers against Gag. So far, no information on which role HERV-K10 plays in the development of this tumor exists.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
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Created: Wed, 19 Oct 2011, 12:29:53 EST by Marion Buck on behalf of Royal Brisbane Clinical School