Fetal progenitor cells naturally transferred through pregnancy participate in inflammation and angiogenesis during wound healing

Nassar, Dany, Droitcourt, Catherine, Mathieu-d'Argent, Emmanuelle, Kim, Min Ji, Khosrotehrani, Kiarash and Aractingi, Selim (2012) Fetal progenitor cells naturally transferred through pregnancy participate in inflammation and angiogenesis during wound healing. The FASEB Journal, 26 1: 149-157. doi:10.1096/fj.11-180695


Author Nassar, Dany
Droitcourt, Catherine
Mathieu-d'Argent, Emmanuelle
Kim, Min Ji
Khosrotehrani, Kiarash
Aractingi, Selim
Title Fetal progenitor cells naturally transferred through pregnancy participate in inflammation and angiogenesis during wound healing
Journal name The FASEB Journal   Check publisher's open access policy
ISSN 0892-6638
1530-6860
Publication date 2012-01
Year available 2011
Sub-type Article (original research)
DOI 10.1096/fj.11-180695
Volume 26
Issue 1
Start page 149
End page 157
Total pages 9
Place of publication Bethesda, MD, United States
Publisher Federation of American Societies for Experimental Biology
Collection year 2012
Language eng
Formatted abstract
The phenotype and fate of fetal microchimeric cells transfered into the maternal circulation during pregnancy are not well described. Since progenitors from distal sites mobilize during wound healing, we analyzed the recruitment and plasticity of fetal progenitors into maternal wounds. Wounds were generated on normal and bleomycin-induced fibrotic skin of parous or pregnant wild-type females with fluorescent GFP+ fetuses. Analyses were performed on skin and blood specimens through PCR, immunohistochemistry, and flow cytometry. Controls consisted of parous and pregnant females without wounds and virgin females with wounds. Fetal cells were detected in all skin specimens of parous mice as long as healing was not achieved. During early stages of wound healing, fetal cells expressed mainly leukocyte markers, while in later phases endothelial markers prevailed. Fetally derived vessels connected to maternal circulation were also found, demonstrating the transfer of fetal endothelial progenitor cells. Wounding mobilized fetal CD34+ckit− cells into the blood during pregnancy. Most of this population was CD11bVEGFR2. Another part was CD11b+ with a fraction expressing VEGFR2. VEGFa-spiked Matrigel plugs partially mimicked this fetal progenitor recruitment and mobilization into the blood. In summary, fetal cells that mobilize in response to wounding are mainly progenitor cells and participate in angiogenesis and inflammation.—Nassar, D., Droitcourt, C., Mathieu-d'Argent, E., Kim, M. J., Khosrotehrani, K., Aractingi, S. Fetal progenitor cells naturally transferred through pregnancy participate in inflammation and angiogenesis during wound healing.
Keyword Microchimerism
Skin
VEGFa
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online before print October 5, 2011

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2012 Collection
School of Medicine Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 17 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 22 times in Scopus Article | Citations
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Created: Wed, 19 Oct 2011, 12:01:04 EST by Dr Kiarash Khosrotehrani on behalf of Medicine - Princess Alexandra Hospital