Alkylamides from echinacea modulate induced immune responses in macrophages

Matthias, A., Banbury, L., Stevenson, L. M., Bone, K. M., Leach, D. N. and Lehmann, R. P. (2007) Alkylamides from echinacea modulate induced immune responses in macrophages. Immunological Investigations, 36 2: 117-130. doi:10.1080/08820130600745786

Author Matthias, A.
Banbury, L.
Stevenson, L. M.
Bone, K. M.
Leach, D. N.
Lehmann, R. P.
Title Alkylamides from echinacea modulate induced immune responses in macrophages
Journal name Immunological Investigations   Check publisher's open access policy
ISSN 0882-0139
Publication date 2007
Sub-type Article (original research)
DOI 10.1080/08820130600745786
Volume 36
Issue 2
Start page 117
End page 130
Total pages 14
Place of publication New York, United States
Publisher Informa Healthcare
Language eng
Abstract The ability of Echinacea and its components to alter the immune response was examined in vitro in a macrophage cell line under either basal or immunostimulated conditions. Potential immunostimulatory and inflammatory activity was determined using a nuclear transcription factor (NFκB) expression, tumour necrosis factor α (TNFα) and nitric oxide (NO) production as biomarkers. In the absence of alternate stimulation, the only significant effects seen were a decrease in NFκB expression by a 2-ene alkylamide ((2E)-N-isobutylundeca-2-ene-8,10-diynamide (1)) and a decrease in TNFα levels by cichoric acid and an Echinacea alkylamide fraction (EPL AA). When the cells were stimulated by lipopolysaccharide (LPS), inhibition of the increased NFκB expression levels was caused by cichoric acid, an Echinacea preparation (EPL), EPL AA and a 2,4-diene ((2E,4E,8Z,10Z)-N-isobutyldodeca-2,4,8,10-tetraenamide (2)). Increases in TNFα levels were inhibited by cichoric acid, EPL and EPL AA but enhanced by 1 in the presence of LPS, while only EPL AA was able to inhibit the stimulated increases in NO. When using phorbol myristate acetate to stimulate the cells, NFκB and NO levels were unaffected by Echinacea or its components while only cichoric acid and 2 inhibited TNFα levels. Although cichoric acid was found to have an effect, it is probably not an important contributor to the Echinacea modulation of the immune response in vivo, as it is not bioavailable. Echinacea appears to attenuate the response of macrophages to an immune stimulus and its combination of phytochemicals exhibits different pharmacological properties to one or more of the isolated major individual components.
Keyword Echinacea
Immune modulation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
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Created: Wed, 19 Oct 2011, 09:24:13 EST by Anita Matthias on behalf of School of Biomedical Sciences