Expression of multiple glutamate transporter splice variants in the rodent testis

Lee, Aven, Anderson, Ashley R., Barnett, Amanda C., Chan, Anthony and Pow, David V. (2011) Expression of multiple glutamate transporter splice variants in the rodent testis. Asian Journal of Andrology, 13 2: 254-265. doi:10.1038/aja.2010.99

Author Lee, Aven
Anderson, Ashley R.
Barnett, Amanda C.
Chan, Anthony
Pow, David V.
Title Expression of multiple glutamate transporter splice variants in the rodent testis
Journal name Asian Journal of Andrology   Check publisher's open access policy
ISSN 1008-682X
Publication date 2011-03
Year available 2010
Sub-type Article (original research)
DOI 10.1038/aja.2010.99
Open Access Status DOI
Volume 13
Issue 2
Start page 254
End page 265
Total pages 12
Place of publication New York, United States
Publisher Nature Publishing Group
Collection year 2012
Language eng
Abstract Glutamate is a regulated molecule in the mammalian testis. Extracellular regulation of glutamate in the body is determined largely by the expression of plasmalemmal glutamate transporters. We have examined by PCR, western blotting and immunocytochemistry the expression of a panel of sodium-dependent plasmalemmal glutamate transporters in the rat testis. Proteins examined included: glutamate aspartate transporter (GLAST), glutamate transporter 1 (GLT1), excitatory amino acid carrier 1 (EAAC1), excitatory amino acid transporter 4 (EAAT4) and EAAT5. We demonstrate that many of the glutamate transporters in the testis are alternately spliced. GLAST is present as exon-3- and exon-9-skipping forms. GLT1 was similarly present as the alternately spliced forms GLT1b and GLT1c, whereas the abundant brain form (GLT1a) was detectable only at the mRNA level. EAAT5 was also strongly expressed, whereas EAAC1 and EAAT4 were absent. These patterns of expression were compared with the patterns of endogenous glutamate localization and with patterns of d-aspartate accumulation, as assessed by immunocytochemistry. The presence of multiple glutamate transporters in the testis, including unusually spliced forms, suggests that glutamate homeostasis may be critical in this organ. The apparent presence of many of these transporters in the testis and sperm may indicate a need for glutamate transport by such cells.
Keyword Excitatory amino acid transporter
Glutamate aspartate transporter
Glutamate transporter 1
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online 20 December 2010

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2012 Collection
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Citation counts: TR Web of Science Citation Count  Cited 6 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 6 times in Scopus Article | Citations
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Created: Wed, 19 Oct 2011, 08:58:00 EST by Roheen Gill on behalf of UQ Centre for Clinical Research