Characterization and use of the NSC-34 cell line for study of neurotrophin receptor trafficking

Matusica, Dusan, Fenech, Matthew P., Rogers, Mary-Louise and Rush, Robert A. (2008) Characterization and use of the NSC-34 cell line for study of neurotrophin receptor trafficking. Journal of Neuroscience Research, 86 3: 553-565. doi:10.1002/jnr.21507


Author Matusica, Dusan
Fenech, Matthew P.
Rogers, Mary-Louise
Rush, Robert A.
Title Characterization and use of the NSC-34 cell line for study of neurotrophin receptor trafficking
Journal name Journal of Neuroscience Research   Check publisher's open access policy
ISSN 0360-4012
1097-4547
Publication date 2008-02-15
Year available 2007
Sub-type Article (original research)
DOI 10.1002/jnr.21507
Volume 86
Issue 3
Start page 553
End page 565
Total pages 13
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Language eng
Abstract This study addressed the suitability of the NSC-34 cell line as a motor neuron-like model for investigating neurotrophin receptor trafficking and associated subcellular processes. Initially, culture conditions were optimized for the use of NSC-34 cells in confocal microscopy. Cell surface markers, as well as markers associated with the regulated endosomal pathway thought to be associated with neurotrophin receptor transport, were identified. The study revealed the presence of a number of molecules previously not described in the literature, including the tropomyosin-like receptor kinase C (TrkC), sortilin, the vesicular acetylcholine transporter (VAChT), and the lipid raft-associated ganglioside GT1b. The presence of both sortilin and Gt1b was of special interest, insofar as these markers have been implicated in direct relationships with the p75NTR receptor. Evidence is provided for neurotrophin-dependent internalization of p75NTR and TrkB. Both nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) increased the rate of internalization of p75NTR, with internalization dynamics comparable to those described for other cell lines. Thus, these studies not only describe components of the regulatory process governing the trafficking of this important receptor but also clearly demonstrate the value of NSC-34 cells as a suitable motor neuron model for the study of internalization and trafficking of cell surface molecules.
Keyword Motor neuron
Neurotrophin receptors
Receptor endocytosis
Trafficking
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Article first published online: 26 SEP 2007

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
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Created: Wed, 19 Oct 2011, 08:48:38 EST by Dusan Matusica on behalf of Queensland Brain Institute