The role of miR-124a in early development of the Xenopus eye

Qiu, Rong, Liu, Kaili, Liu, Ying, Mo, Weichuan, Flynt, Alex S., Patton, James G., Kar, Amar, Wu, Jane Y. and He, Rongqiao (2009) The role of miR-124a in early development of the Xenopus eye. Mechanisms of Development, 126 10: 804-816. doi:10.1016/j.mod.2009.08.002

Author Qiu, Rong
Liu, Kaili
Liu, Ying
Mo, Weichuan
Flynt, Alex S.
Patton, James G.
Kar, Amar
Wu, Jane Y.
He, Rongqiao
Title The role of miR-124a in early development of the Xenopus eye
Formatted title
The role of miR-124a in early development of the Xenopus eye
Journal name Mechanisms of Development   Check publisher's open access policy
ISSN 0925-4773
Publication date 2009-10
Sub-type Article (original research)
DOI 10.1016/j.mod.2009.08.002
Volume 126
Issue 10
Start page 804
End page 816
Total pages 13
Place of publication Shannon, Co. Clare, Ireland
Publisher Elsevier Ireland
Language eng
Formatted abstract
It has been reported that miR-124a is abundant in the central nervous system including the eye, and is related to neurogenesis in several species. However, the role of miR-124a in the eye remains unclear. In this study, we show that the expression of miR-124a in Xenopus laevis begins along the neural fold, including the protruding eye anlagen, at a low level at around stage 18; its expression level gradually increases in the neural tube and the eye as embryos develop into later stages and then maintains at a high level in eye to adult stages. Microinjection of a miR-124a precursor at the 8-cell stage leads to malformation of the optic nerve and optic cup, indicating the importance of maintaining low levels of miR-124a during early embryonic development. In addition, miR-124a overexpression markedly down regulates the expression of its predicted targets Lhx2, Hairy2, Gli3, NeuroD1 and Otx2 in/around the eye anlagen, and the interaction of miR-124a with the 3′ UTR of Lhx2 represses gene expression as shown by luciferase assays. Moreover, excess miR-124a inhibits cell proliferation in the eye of Xenopus embryos during retinogenesis. These results indicate that miR-124a acts as a post-transcriptional regulator in the genetic network controlling eye morphogenesis and neurogenesis. The mechanism of miR-124a’s early interaction with the genetic network may also persist in its later role in the maturing and adult eye and brain.
Keyword miR-124a
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
ERA 2012 Admin Only
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 19 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 19 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 19 Oct 2011, 02:11:23 EST by Weichuan Mo on behalf of Queensland Brain Institute