MiR-124 regulates early neurogenesis in the optic vesicle and forebrain by targeting NeuroD1

Liu, Kaili, Liu, Ying, Mo, Weichuan, Qiu, Rong, Wang, Xiumei, Wu, Jane Y. and He, Rongqiao (2011) MiR-124 regulates early neurogenesis in the optic vesicle and forebrain by targeting NeuroD1. Nucleic Acids Research, 39 7: 2869-2879. doi:10.1093/nar/gkq904


Author Liu, Kaili
Liu, Ying
Mo, Weichuan
Qiu, Rong
Wang, Xiumei
Wu, Jane Y.
He, Rongqiao
Title MiR-124 regulates early neurogenesis in the optic vesicle and forebrain by targeting NeuroD1
Formatted title
MiR-124 regulates early neurogenesis in the optic vesicle and forebrain by targeting NeuroD1
Journal name Nucleic Acids Research   Check publisher's open access policy
ISSN 1362-4962
0305-1048
Publication date 2011-04-01
Year available 2010
Sub-type Article (original research)
DOI 10.1093/nar/gkq904
Open Access Status DOI
Volume 39
Issue 7
Start page 2869
End page 2879
Total pages 11
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Collection year 2012
Language eng
Formatted abstract
MicroRNAs (miRNAs) are involved in the fine control of cell proliferation and differentiation during the development of the nervous system. MiR-124, a neural specific miRNA, is expressed from the beginning of eye development in Xenopus, and has been shown to repress cell proliferation in the optic cup, however, its role at earlier developmental stages is unclear. Here, we show that this miRNA exerts a different role in cell proliferation at the optic vesicle stage, the stage which precedes optic cup formation. We show that miR-124 is both necessary and sufficient to promote cell proliferation and repress neurogenesis at the optic vesicle stage, playing an anti-neural role. Loss of miR-124 upregulates expression of neural markers NCAM, N-tubulin while gain of miR-124 downregulates these genes. Furthermore, miR-124 interacts with a conserved miR-124 binding site in the 3′-UTR of NeuroD1 and negatively regulates expression of the proneural marker NeuroD1, a bHLH transcription factor for neuronal differentiation. The miR-124-induced effect on cell proliferation can be antagonized by NeuroD1. These results reveal a novel regulatory role of miR-124 in neural development and uncover a previously unknown interaction between NeuroD1 and miR-124.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ
Additional Notes First published online: December 3, 2010

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Queensland Brain Institute Publications
 
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Created: Wed, 19 Oct 2011, 12:03:23 EST by Weichuan Mo on behalf of Queensland Brain Institute