On the simulation of enzymatic digest patterns: the fragmentation of oligomeric and polymeric galacturonides by endo-polygalacturonase II

Hunt, Jonathan J., Cameron, Randall G. and Williams, Martin A. K. (2006) On the simulation of enzymatic digest patterns: the fragmentation of oligomeric and polymeric galacturonides by endo-polygalacturonase II. Biochimica et Biophysica Acta - General Subjects, 1760 11: 1696-1703. doi:10.1016/j.bbagen.2006.08.022


Author Hunt, Jonathan J.
Cameron, Randall G.
Williams, Martin A. K.
Title On the simulation of enzymatic digest patterns: the fragmentation of oligomeric and polymeric galacturonides by endo-polygalacturonase II
Journal name Biochimica et Biophysica Acta - General Subjects   Check publisher's open access policy
ISSN 0304-4165
1872-8006
Publication date 2006-11
Sub-type Article (original research)
DOI 10.1016/j.bbagen.2006.08.022
Volume 1760
Issue 11
Start page 1696
End page 1703
Total pages 8
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
A simulation methodology for predicting the time-course of enzymatic digestions is described. The model is based solely on the enzyme's subsite architecture and concomitant binding energies. This allows subsite binding energies to be used to predict the evolution of the relative amounts of different products during the digestion of arbitrary mixtures of oligomeric or polymeric substrates. The methodology has been specifically demonstrated by studying the fragmentation of a population of oligogalacturonides of varying degrees of polymerization, when digested by endo-polygalacturonase II (endo-PG II) from Aspergillus niger.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Mathematics and Physics
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Created: Tue, 18 Oct 2011, 12:21:17 EST by Mr Jonathan Hunt on behalf of Mathematics