Functional monoclonal antibodies to p75 neurotrophin receptor raised in knockout mice

Rogers, Mary Louise, Atmosukarto, Ines, Berhanu, Degu A., Matusica, Dusan, Macardle, Peter and Rush, Robert A. (2006) Functional monoclonal antibodies to p75 neurotrophin receptor raised in knockout mice. Journal of Neuroscience Methods, 158 1: 109-120. doi:10.1016/j.jneumeth.2006.05.022


Author Rogers, Mary Louise
Atmosukarto, Ines
Berhanu, Degu A.
Matusica, Dusan
Macardle, Peter
Rush, Robert A.
Title Functional monoclonal antibodies to p75 neurotrophin receptor raised in knockout mice
Journal name Journal of Neuroscience Methods   Check publisher's open access policy
ISSN 0165-0270
1872-678X
Publication date 2006-11-15
Sub-type Article (original research)
DOI 10.1016/j.jneumeth.2006.05.022
Volume 158
Issue 1
Start page 109
End page 120
Total pages 12
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
In this study, p75NTREXONIII knockout mice were used as immune-naive hosts to produce functional antibodies to human p75NTR. Three monoclonal antibodies were produced and named MLR1, MLR2 and MLR3, and isotyped as IgG1, IgG2a and IgG2a, respectively. MLR1 and MLR2 bound to human p75NTR with higher affinity than the well-characterized ME20.4 in ELISA and also recognized p75NTR present on neurons in both rat and mouse. MLR1 and MLR2 bound to nerves known to express p75NTR following injection into Balb/C mice but not p75NTREXONIII knockout mice, indicating the antibodies are directed against the ligand binding extracellular region absent in knockout mice. Both MLR1 and MLR2 partially blocked NGF induced cell death in a mouse cell-line that expresses p75NTR but not TrKA. Importantly, intracerebroventricular injections indicated MLR2 was internalized within the cell bodies of mouse basal forebrain neurons, further demonstrating that this antibody is biologically active.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
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Created: Tue, 18 Oct 2011, 12:05:32 EST by Dusan Matusica on behalf of Queensland Brain Institute