Electrochemistry and biosensing activity of cytochrome c immobilized in macroporous materials

Wang, Ying, Qian, Kun, Guo, Kai, Kong, Jilie, Marty, Jean-Louis, Yu, Chengzhong and Liu, Baohong (2011) Electrochemistry and biosensing activity of cytochrome c immobilized in macroporous materials. Microchimica Acta, 175 1-2: 87-95. doi:10.1007/s00604-011-0638-8


Author Wang, Ying
Qian, Kun
Guo, Kai
Kong, Jilie
Marty, Jean-Louis
Yu, Chengzhong
Liu, Baohong
Title Electrochemistry and biosensing activity of cytochrome c immobilized in macroporous materials
Journal name Microchimica Acta   Check publisher's open access policy
ISSN 0026-3672
1436-5073
Publication date 2011-10
Sub-type Article (original research)
DOI 10.1007/s00604-011-0638-8
Volume 175
Issue 1-2
Start page 87
End page 95
Total pages 9
Place of publication Vienna, Austria
Publisher Springer Wien
Collection year 2012
Language eng
Formatted abstract
An amperometric biosensor for hydrogen peroxide (H2O2) has been constructed by immobilizing cytochrome c on an indium/tin oxide (ITO) electrode modified with a macroporous material. Cyclic voltammetry showed that the direct and quasi-reversible electron transfer of cytochrome c proceeds without the need for an electron mediator. A surface-controlled electron transfer process can be observed with an apparent heterogeneous electron-transfer rate constant (ks) of 29.2 s−1. The biosensor displays excellent electrocatalytic responses to the reduction of H2O2 to give amperometric responses that increase steadily with the concentration of H2O2 in the range from 5 μM to 2 mM. The detection limit is 0.61 μM at pH 7.4. The apparent Michaelis-Menten constant (Km) of the biosensor is 1.06 mM. This investigation not only provided a method for the direct electron transfer of cytochrome c on macroporous materials, but also established a feasible approach for durable and reliable detection of H2O2.
Keyword Macroporous material
Cytochrome
Hydrogen peroxide
Direct electron transfer
Biosensor
Amperometric hydrogen-peroxide
Central-nervous-system
Horseradish-peroxidase
Carbon electrode
Porous materials
Nanoparticles
Involvement
Proteolysis
Expression
Nanotubes
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Sun, 16 Oct 2011, 00:04:39 EST by System User on behalf of Functional Nanomaterials