A novel adaptor-related protein complex

Simpson, F., Bright, N. A., West, M. A., Newman, L. S., Darnell, R. B. and Robinson, M. S. (1996) A novel adaptor-related protein complex. Journal of Cell Biology, 133 4: 749-760. doi:10.1083/jcb.133.4.749

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Author Simpson, F.
Bright, N. A.
West, M. A.
Newman, L. S.
Darnell, R. B.
Robinson, M. S.
Title A novel adaptor-related protein complex
Journal name Journal of Cell Biology   Check publisher's open access policy
ISSN 0021-9525
Publication date 1996-05-19
Sub-type Article (original research)
DOI 10.1083/jcb.133.4.749
Open Access Status File (Publisher version)
Volume 133
Issue 4
Start page 749
End page 760
Total pages 12
Place of publication New York, NY, United States
Publisher Rockefeller University Press
Language eng
Abstract Coat proteins are required for the budding of the transport vesicles that mediate membrane traffic pathways, but for many pathways such proteins have not yet been identified. We have raised antibodies against p47, a homologue of the medium chains of the adaptor complexes of clathrin-coated vesicles, to determine whether this protein might be a component of a new type of coat. p47 coimmunoprecipitates with three other proteins: two unknown proteins of 160 and 25 kD, and β-NAP, a homologue of the β/β′-adaptins, indicating that it is a subunit of an adaptor-like heterotetrameric complex. However, p47 is not enriched in preparations of clathrin-coated vesicles. Recruitment of the p47-containing complex onto cell membranes is stimulated by GTPγS and blocked by brefeldin A, indicating that, like other coat proteins, its membrane association is regulated by an ARF. The newly recruited complex is localized to non-clathrin-coated buds and vesicles associated with the TGN. Endogenous complex in primary cultures of neuronal cells is also localized to the TGN, and in addition, some complex is associated with the plasma membrane. These results indicate that the complex is a component of a novel type of coat that facilitates the budding of vesicles from the TGN, possibly for transporting newly synthesized proteins to the plasma membrane.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
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Created: Fri, 14 Oct 2011, 12:23:21 EST by Dr Fiona Simpson on behalf of School of Chemistry & Molecular Biosciences