A new bioproduction route for a novel antimicrobial peptide

Tay, Derrick Kok Sing, Rajagopalan, Gobinath, Li, Xiang, Chen, Yu, Linda H.L. Lua and Leong, Susanna Su Jan (2011) A new bioproduction route for a novel antimicrobial peptide. Biotechnology and Bioengineering, 108 3: 572-581. doi:10.1002/bit.22970


Author Tay, Derrick Kok Sing
Rajagopalan, Gobinath
Li, Xiang
Chen, Yu
Linda H.L. Lua
Leong, Susanna Su Jan
Title A new bioproduction route for a novel antimicrobial peptide
Journal name Biotechnology and Bioengineering   Check publisher's open access policy
ISSN 0006-3592
Publication date 2011-03
Year available 2010
Sub-type Article (original research)
DOI 10.1002/bit.22970
Volume 108
Issue 3
Start page 572
End page 581
Total pages 10
Place of publication United States
Publisher John Wiley & Sons, Inc.
Collection year 2012
Language eng
Abstract Beta defensins are antimicrobial peptides (AMPs) with a broad spectrum antimicrobial behavior against pathogens while having minimal tendency to incur pathogen resistance. Human β-defensin 28 (hBD28) is a strongly cationic AMP and hence hypothesized to be highly effective in permeabilizing negatively-charged pathogen membranes. However, the scarcity of hBD28 in vivo has impeded detailed structure and antimicrobial studies of hBD28. Chemical synthesis of hBD28 rendered extremely poor yields due to inefficient cysteine oxidation. In this study, a rapid and scalable production route to produce bioactive hBD28 in Escherichia coli (E. coli) is reported. The design of a dual fusion tag expression construct was pivotal in enhancing soluble expression and easing purification of hBD28. The final hBD28 (purity >95%) displayed significant antimicrobial activity against E. coli K12 and showed dose-dependent killing kinetics. Circular dichroism spectroscopy confirmed the presence of both β-sheet and α-helix conformations in the secondary structure of hBD28.
Keyword Antimicrobial activity
Antimicrobial peptide
Bioprocess
E. coli
Human β-defensin 28
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Fri, 14 Oct 2011, 08:55:22 EST by Linda Lua on behalf of Aust Institute for Bioengineering & Nanotechnology