Long-term stability of adipose tissue generated from a vascularized pedicled fat flap inside a chamber

Doldere, Juergan H., Thompson, Erik W., Slavin, John, Trost, Nicholas, Cooper-White, Justin J., Cao, Yang, O'Connor, Andrea J., Penington, Anthony, Morrison, Wayne A. and Abberton, Keren M. (2011) Long-term stability of adipose tissue generated from a vascularized pedicled fat flap inside a chamber. Plastic and Reconstructive Surgery, 127 6: 2283-2292. doi:10.1097/PRS.0b013e3182131c3e

Author Doldere, Juergan H.
Thompson, Erik W.
Slavin, John
Trost, Nicholas
Cooper-White, Justin J.
Cao, Yang
O'Connor, Andrea J.
Penington, Anthony
Morrison, Wayne A.
Abberton, Keren M.
Title Long-term stability of adipose tissue generated from a vascularized pedicled fat flap inside a chamber
Journal name Plastic and Reconstructive Surgery   Check publisher's open access policy
ISSN 0032-1052
Publication date 2011-06
Sub-type Article (original research)
DOI 10.1097/PRS.0b013e3182131c3e
Volume 127
Issue 6
Start page 2283
End page 2292
Total pages 10
Place of publication Baltimore, MD, U.S.A.
Publisher Lippincott Williams & Wilkins
Collection year 2012
Language eng
Formatted abstract
Background: Numerous studies demonstrate the generation and short-term survival of adipose tissue; however, long-term persistence remains elusive. This study evaluates long-term survival and transferability of de novo adipose constructs based on a ligated vascular pedicle and tissue engineering chamber combination.

Methods: Defined adipose tissue flaps were implanted into rats in either intact or perforated domed chambers. In half of the groups, the chambers were removed after 10 weeks and the constructs transferred on their vascular pedicle to a new site, where they were observed for a further 10 weeks. In the remaining groups, the tissue construct was observed for 20 weeks inside the chamber. Tissue volume was assessed using magnetic resonance imaging and histologic measures, and constructs were assessed for stability and necrosis. Sections were assessed histologically and for proliferation using Ki-67.

Results: At 20 weeks, volume analysis revealed an increase in adipose volume from 0.04 ± 0.001 ml at the time of insertion into the chambers to 0.27 ± 0.004 ml in the closed and 0.44 ± 0.014 ml in the perforated chambers. There was an additional increase of approximately 10 to 15 percent in tissue volume in flaps that remained in chambers for 20 weeks, whereas the volume of the transferred tissue not in chambers remained unaltered. Histomorphometric assessment of the tissues documented no signs of hypertrophy, fat necrosis, or atypical changes of the newly generated tissue.

Conclusion: This study presents a promising new method of generating significant amounts of mature, vascularized, stable, and transferable adipose tissue for permanent autologous soft-tissue replacement.
Keyword Growth-factor-I
Autologous fat
Reconstructive surgery
PLGA/PEG microspheres
Breast augmentation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Note: First Author's name "Juergan H. Dolderer" misspelt.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
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Created: Fri, 14 Oct 2011, 08:36:47 EST by Justin Cooper-White on behalf of Aust Institute for Bioengineering & Nanotechnology