Modulation of collagen II fiber formation in 3-D porous scaffold environments

Tan, Guak-Kim, Dinnes, Donna Lee M. and Cooper-White, Justin J. (2011) Modulation of collagen II fiber formation in 3-D porous scaffold environments. Acta Biomaterialia, 7 7: 2804-2816. doi:10.1016/j.actbio.2011.03.022

Author Tan, Guak-Kim
Dinnes, Donna Lee M.
Cooper-White, Justin J.
Title Modulation of collagen II fiber formation in 3-D porous scaffold environments
Journal name Acta Biomaterialia   Check publisher's open access policy
ISSN 1742-7061
Publication date 2011-07
Sub-type Article (original research)
DOI 10.1016/j.actbio.2011.03.022
Volume 7
Issue 7
Start page 2804
End page 2816
Total pages 13
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2012
Language eng
Abstract Collagen II, a major extracellular matrix component in cartilaginous tissues, undergoes fibrillogenesis under physiological conditions. The present study explored collagen II fiber formation in solution and in two- (coverslip) and three-dimensional (scaffold) environments under different incubation conditions. These conditions include variations in adsorption buffers, the presence of 1-ethyl-3-(3-dimenthylaminopropyl) carbodiimide/N-hydroxysuccinimide crosslinker and the nature of the material surfaces. We extend our observations of collagen II fiber formation in two dimensions to develop an approach for the formation of a fibrillar collagen II network throughout surface-modified polylactide-co-glycolide porous scaffolds. Morphologically, the collagen II network is similar to that present in native articular cartilage. Biological validation of the resultant optimized functional scaffold, using rat bone marrow-derived mesenchymal stem cells, shows appreciable cell infiltration throughout the scaffold with enhanced cell spreading at 24 h post-seeding. This economic and versatile approach is thus believed to have significant potential in cartilage tissue engineering applications.
Keyword Collagen II
EDAC/NHS crosslinking
2-D and 3-D environments
Surface modification
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

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Created: Fri, 14 Oct 2011, 08:31:58 EST by Justin Cooper-White on behalf of Aust Institute for Bioengineering & Nanotechnology