One-step homogeneous C-reactive protein assay for saliva

Punyadeera, Chamindie, Dimeski, Goce, Kostner, Karam, Beyerlein, Peter and Cooper-White, Justin (2011) One-step homogeneous C-reactive protein assay for saliva. Journal of Immunological Methods, 373 1-2: 19-25. doi:10.1016/j.jim.2011.07.013

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Author Punyadeera, Chamindie
Dimeski, Goce
Kostner, Karam
Beyerlein, Peter
Cooper-White, Justin
Title One-step homogeneous C-reactive protein assay for saliva
Journal name Journal of Immunological Methods   Check publisher's open access policy
ISSN 0022-1759
Publication date 2011-10-28
Sub-type Article (original research)
DOI 10.1016/j.jim.2011.07.013
Volume 373
Issue 1-2
Start page 19
End page 25
Total pages 7
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2012
Language eng
Formatted abstract
Background: Cardiovascular disease is the leading cause of death in the world. Human C-reactive protein (CRP) has been used in the risk assessment of coronary events. Human saliva mirrors the body's health and well-being and is non-invasive, easy to collect and ideal for third world countries as well as for large patient screening. The aim was to establish a saliva CRP reference range and to demonstrate the clinical utility of salivary CRP levels in assessing the coronary events in a primary health care setting.

Methods: We have used a homogeneous bead based assay to detect CRP levels in human saliva. We have developed a rapid 15 min (vs 90 min), sequential, one-step assay to detect CRP in saliva. Saliva was collected from healthy volunteers (n = 55, ages 20–70 years) as well as from cardiac patients (n = 28, ages 43–86 years).

Results: The assay incubation time was optimised from 90 min to 15 min and generated a positive correlation (n = 29, range 10–2189 pg/mL, r2 = 0.94; Passing Bablok slope 0.885, Intercept 0, p > 0.10), meaning we could decrease the incubation time and produce equivalent results with confidence. The mean CRP level in the saliva of healthy human volunteers was 285 pg/mL and in cardiac patients was 1680 pg/mL (p < 0.01). Analysis of CRP concentrations in paired serum and saliva samples from cardiac patients gave a positive correlation (r2 = 0.84, p < 0.001) and the salivary CRP concentration capable of distinguishing healthy from diseased patients.

Conclusions: The results suggest that this minimally invasive, rapid and sensitive assay will be useful in large patient screening studies for risk assessment of coronary events.

Highlights: ► We have developed 15-min salivary CRP assay compatible for near patient testing. ► Salivary CRP levels are significantly higher in cardiac patients compared to healthy individuals. ► Salivary CRP levels are useful in risk assessment of coronary events.
Keyword Human saliva
High-sensitive C-reactive protein assay
Homogeneous bead based assay
Cardiovascular risk factors
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

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Created: Fri, 14 Oct 2011, 08:25:55 EST by Justin Cooper-White on behalf of School of Chemical Engineering