Usefulness of published PCR primers in detecting human rhinovirus infection

Faux, Cassandra E., Arden, Katherine E., Lambert, Stephen B., Nissen, Michael D., Nolan, Terry M., Chang, Anne B., Sloots, Theo P. and Mackay, Ian M. (2011) Usefulness of published PCR primers in detecting human rhinovirus infection. Emerging Infectious Diseases, 17 2: 296-298. doi:10.3201/eid1702.101123

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Author Faux, Cassandra E.
Arden, Katherine E.
Lambert, Stephen B.
Nissen, Michael D.
Nolan, Terry M.
Chang, Anne B.
Sloots, Theo P.
Mackay, Ian M.
Title Usefulness of published PCR primers in detecting human rhinovirus infection
Journal name Emerging Infectious Diseases   Check publisher's open access policy
ISSN 1080-6040
Publication date 2011-02
Sub-type Article (original research)
DOI 10.3201/eid1702.101123
Open Access Status DOI
Volume 17
Issue 2
Start page 296
End page 298
Total pages 3
Place of publication Atlanta, GA, U.S.A.
Publisher U.S. Department of Health and Human Services
Collection year 2012
Language eng
Formatted abstract
We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)–specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology.
Keyword Polymerase chain-reaction
Respiratory viruses
Clinical specimens
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

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Created: Thu, 13 Oct 2011, 20:47:48 EST by System User on behalf of School of Medicine