Correlation of second virial coefficient with solubility for proteins in salt solutions

Mehta, Chirag M., White, Edward T. and Litster, James D. (2012) Correlation of second virial coefficient with solubility for proteins in salt solutions. Biotechnology Progress, 28 1: 163-170. doi:10.1002/btpr.724

Author Mehta, Chirag M.
White, Edward T.
Litster, James D.
Title Correlation of second virial coefficient with solubility for proteins in salt solutions
Journal name Biotechnology Progress   Check publisher's open access policy
ISSN 8756-7938
Publication date 2012-01
Year available 2011
Sub-type Article (original research)
DOI 10.1002/btpr.724
Volume 28
Issue 1
Start page 163
End page 170
Total pages 8
Place of publication Hoboken, NJ, United States
Publisher Wiley-Blackwell Publishing
Collection year 2012
Language eng
Formatted abstract
In this work, osmotic second virial coefficients (B22) were determined and correlated with the measured solubilities for the proteins, α-amylase, ovalbumin, and lysozyme. The B22 values and solubilities were determined in similar solution conditions using two salts, sodium chloride and ammonium sulfate in an acidic pH range. An overall decrease in the solubility of the proteins (salting out) was observed at high concentrations of ammonium sulfate and sodium chloride solutions. However, for α-amylase, salting-in behavior was also observed in low concentration sodium chloride solutions. In ammonium sulfate solutions, the B22 are small and close to zero below 2.4 M. As the ammonium sulfate concentrations were further increased, B22 values decreased for all systems studied. The effect of sodium chloride on B22 varies with concentration, solution pH, and the type of protein studied. Theoretical models show a reasonable fit to the experimental derived data of B22 and solubility. B22 is also directly proportional to the logarithm of the solubility values for individual proteins in salt solutions, so the log-linear empirical models developed in this work can also be used to rapidly predict solubility and B22 values for given protein–salt systems.
Keyword Solubility
Osmotic second virial coefficient
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article first published online: 14 OCT 2011

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Chemical Engineering Publications
Official 2012 Collection
Advanced Water Management Centre Publications
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Citation counts: TR Web of Science Citation Count  Cited 9 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 11 times in Scopus Article | Citations
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Created: Thu, 13 Oct 2011, 14:05:36 EST by Mr Chirag Mehta on behalf of Advanced Water Management Centre