Cells respond to mechanical stress by rapid disassembly of caveolae

Sinha, Bidisha, Koster, Darius, Ruez, Richard, Gonnord, Pauline, Bastiani, Michele, Abankwa, Daniel, Stan, Radu V., Butler-Browne, Gillian, Vedie, Benoit, Johannes, Ludger, Morone, Nobuhiro, Parton, Robert G., Raposo, Graca, Sens, Pierre, Lamaze, Christophe and Nassoy, Pierre (2011) Cells respond to mechanical stress by rapid disassembly of caveolae. Cell, 144 3: 402-413. doi:10.1016/j.cell.2010.12.031

Author Sinha, Bidisha
Koster, Darius
Ruez, Richard
Gonnord, Pauline
Bastiani, Michele
Abankwa, Daniel
Stan, Radu V.
Butler-Browne, Gillian
Vedie, Benoit
Johannes, Ludger
Morone, Nobuhiro
Parton, Robert G.
Raposo, Graca
Sens, Pierre
Lamaze, Christophe
Nassoy, Pierre
Title Cells respond to mechanical stress by rapid disassembly of caveolae
Journal name Cell   Check publisher's open access policy
ISSN 0092-8674
Publication date 2011-02-04
Sub-type Article (original research)
DOI 10.1016/j.cell.2010.12.031
Volume 144
Issue 3
Start page 402
End page 413
Total pages 12
Place of publication United States
Publisher Cell Press
Collection year 2012
Language eng
Abstract The functions of caveolae, the characteristic plasma membrane invaginations, remain debated. Their abundance in cells experiencing mechanical stress led us to investigate their role in membrane-mediated mechanical response. Acute mechanical stress induced by osmotic swelling or by uniaxial stretching results in a rapid disappearance of caveolae, in a reduced caveolin/Cavin1 interaction, and in an increase of free caveolins at the plasma membrane. Tether-pulling force measurements in cells and in plasma membrane spheres demonstrate that caveola flattening and disassembly is the primary actin- and ATP-independent cell response that buffers membrane tension surges during mechanical stress. Conversely, stress release leads to complete caveola reassembly in an actin- and ATP-dependent process. The absence of a functional caveola reservoir in myotubes from muscular dystrophic patients enhanced membrane fragility under mechanical stress. Our findings support a new role for caveolae as a physiological membrane reservoir that quickly accommodates sudden and acute mechanical stresses.
Keyword Signal-regulated kinase
Membrane tension
Dependent activation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Institute for Molecular Bioscience - Publications
Centre for Microscopy and Microanalysis Publications
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Citation counts: TR Web of Science Citation Count  Cited 184 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 13 Oct 2011, 12:29:31 EST by Mrs Michele Bastiani on behalf of Institute for Molecular Bioscience