Microfluidic device for single-molecule experiments with enhanced photostability

Lemke, Edward A., Gambin, Yann, Vandelinder, Virginia, Brustad, Eric M., Liu, Hsiao-Wei, Schultz, Peter G., Groisman, Alex and Deniz, Ashok A. (2009) Microfluidic device for single-molecule experiments with enhanced photostability. Journal of the American Chemical Society, 131 38: 13610-13612. doi:10.1021/ja9027023


Author Lemke, Edward A.
Gambin, Yann
Vandelinder, Virginia
Brustad, Eric M.
Liu, Hsiao-Wei
Schultz, Peter G.
Groisman, Alex
Deniz, Ashok A.
Title Microfluidic device for single-molecule experiments with enhanced photostability
Journal name Journal of the American Chemical Society   Check publisher's open access policy
ISSN 0002-7863
1943-2984
1520-5126
Publication date 2009-09-30
Sub-type Article (original research)
DOI 10.1021/ja9027023
Volume 131
Issue 38
Start page 13610
End page 13612
Total pages 3
Place of publication Washington, DC, United States
Publisher American Chemical Society
Language eng
Abstract A microfluidic device made of polydimethylsiloxane (PDMS) addresses key limitations in single-molecule fluorescence experiments by providing high dye photostability and low sample sticking. Photobleaching is dramatically reduced by deoxygenation via gas diffusion through porous channel walls. Rapid buffer exchange in a laminar sheath flow followed by optical interrogation minimizes surface−sample contacts and allows the in situ addition and combination of other reagents.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: ERA 2012 Admin Only
Institute for Molecular Bioscience - Publications
 
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Created: Thu, 13 Oct 2011, 12:14:02 EST by Yann Gambin on behalf of Institute for Molecular Bioscience