Contribution of siderophore systems to growth and urinary tract colonization of asymptomatic bacteriuria Escherichia coli

Watts, Rebecca E., Totsika, Makrina, Challinor, Victoria L., Mabbett, Amanda N., Ulett, Glen C., De Voss, James J. and Schembri, Mark A. (2012) Contribution of siderophore systems to growth and urinary tract colonization of asymptomatic bacteriuria Escherichia coli. Infection and Immunity, 80 1-2: 333-344. doi:10.1128/IAI.05594-11

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Author Watts, Rebecca E.
Totsika, Makrina
Challinor, Victoria L.
Mabbett, Amanda N.
Ulett, Glen C.
De Voss, James J.
Schembri, Mark A.
Title Contribution of siderophore systems to growth and urinary tract colonization of asymptomatic bacteriuria Escherichia coli
Formatted title
Contribution of siderophore systems to growth and urinary tract colonization of asymptomatic bacteriuria Escherichia coli
Journal name Infection and Immunity   Check publisher's open access policy
ISSN 1098-5522
1070-6313
Publication date 2012-01
Year available 2011
Sub-type Article (original research)
DOI 10.1128/IAI.05594-11
Open Access Status File (Publisher version)
Volume 80
Issue 1-2
Start page 333
End page 344
Total pages 12
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2012
Language eng
Abstract The molecular mechanisms that define asymptomatic bacteriuria (ABU) E. coli colonization of the human urinary tract remain to be properly elucidated. Here we utilize ABU E. coli strain 83972 as a model to dissect the contribution of siderophores to iron acquisition, growth, fitness and colonization of the urinary tract. We show that E. coli 83972 produces enterobactin, salmochelin, aerobactin and yersiniabactin, and examine the role of these systems using mutants defective in siderophore biosynthesis and uptake. Enterobactin and aerobactin contributed most to total siderophore activity and growth in defined iron-deficient media. No siderophores were detected in an 83972 quadruple mutant deficient in all four siderophore biosynthesis pathways; this mutant did not grow in defined iron-deficient media but grew in iron-limited pooled human urine due to iron uptake via the FecA ferric citrate receptor. In a mixed 1:1 growth assay with 83972 there was no fitness disadvantage of the 83972 quadruple biosynthetic mutant, demonstrating its capacity to act as a ‘cheater’ and utilize siderophores produced by the wild-type strain for iron uptake. An 83972 enterobactin/salmochelin double receptor mutant was outcompeted by 83972 in human urine and the mouse urinary tract, indicating a role for catecholate receptors in urinary tract colonization.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes First published September 2011 (online)

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Thu, 13 Oct 2011, 12:12:10 EST by Assoc Prof Mark Schembri on behalf of School of Chemistry & Molecular Biosciences