Multicolor single-molecule FRET to explore protein folding and binding

Gambin, Yann and Deniz, Ashok A. (2010) Multicolor single-molecule FRET to explore protein folding and binding. Molecular Biosystems, 6 9: 1540-1547. doi:10.1039/c003024d


Author Gambin, Yann
Deniz, Ashok A.
Title Multicolor single-molecule FRET to explore protein folding and binding
Journal name Molecular Biosystems   Check publisher's open access policy
ISSN 1742-206X
1742-2051
Publication date 2010
Sub-type Article (original research)
DOI 10.1039/c003024d
Open Access Status Not Open Access
Volume 6
Issue 9
Start page 1540
End page 1547
Total pages 8
Place of publication Cambridge, United Kingdom
Publisher Royal Society of Chemistry
Language eng
Abstract Proper protein function in cells, tissues and organisms depends critically on correct protein folding or interaction with partners. Over the last decade, single-molecule FRET (smFRET) has emerged as a powerful tool to probe complex distributions, dynamics, pathways and landscapes in protein folding and binding reactions, leveraging its ability to avoid averaging over an ensemble of molecules. While smFRET was practiced in a two-color form until recently, the last few years have seen the development of enhanced multicolor smFRET methods that provide additional structural information permitting us to probe more complex mechanisms. In this review, we provide a brief introduction to the smFRET technique, then follow with advanced multicolor measurements and end with ongoing methodology developments in microfluidics and proteinlabeling that are beginning to make these techniques more broadly applicable to answering a number of key questions about folding and binding.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: ERA 2012 Admin Only
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 32 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 35 times in Scopus Article | Citations
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Created: Thu, 13 Oct 2011, 11:26:33 EST by Yann Gambin on behalf of Institute for Molecular Bioscience