Molecular characterization of UpaB and UpaC, two new autotransporter proteins of uropathogenic Escherichia coli CFT073

Allsopp, Luke P., Beloin, Christophe, Ulett, Glen C., Valle, Jaione, Totsika, Makrina, Sherlock, Orla, Ghigo, Jean-Marc and Schembri, Mark A. (2012) Molecular characterization of UpaB and UpaC, two new autotransporter proteins of uropathogenic Escherichia coli CFT073. Infection and Immunity, 80 1: 321-332. doi:10.1128/IAI.05322-11

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Author Allsopp, Luke P.
Beloin, Christophe
Ulett, Glen C.
Valle, Jaione
Totsika, Makrina
Sherlock, Orla
Ghigo, Jean-Marc
Schembri, Mark A.
Title Molecular characterization of UpaB and UpaC, two new autotransporter proteins of uropathogenic Escherichia coli CFT073
Formatted title
Molecular characterization of UpaB and UpaC, two new autotransporter proteins of uropathogenic Escherichia coli CFT073
Journal name Infection and Immunity   Check publisher's open access policy
ISSN 1098-5522
1070-6313
Publication date 2012-01
Year available 2011
Sub-type Article (original research)
DOI 10.1128/IAI.05322-11
Open Access Status File (Publisher version)
Volume 80
Issue 1
Start page 321
End page 332
Total pages 12
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2012
Language eng
Formatted abstract
Uropathogenic Escherichia coli (UPEC) is the primary cause of urinary tract infection (UTI) in the developed world. The major factors associated with virulence of UPEC are fimbrial adhesins, which mediate specific attachment to host receptors and trigger innate host responses. Another group of adhesins is represented by the autotransporter (AT) subgroup of proteins. The genome sequenced prototype UPEC strain CFT073 contains 11 putative AT-encoding genes. In this study we have performed a detailed molecular characterization of two closely related AT adhesins from CFT073: UpaB (c0426) and UpaC (c0478). PCR screening revealed that the upaB and upaC AT-encoding genes are common in E. coli. The upaB and upaC genes were cloned and characterized in a recombinant E. coli K-12 strain background. This revealed that they encode proteins located at the cell surface but possess different functional properties; UpaB mediates adherence to several ECM proteins while UpaC expression is associated with increased biofilm formation. In CFT073, upaB is expressed while upaC is transcriptionally repressed by the global regulator H-NS. In competitive colonization experiments employing the mouse UTI model, CFT073 significantly out-competed its upaB (but not upaC) isogenic mutant strain in the bladder. This attenuated phenotype was also observed in single challenge experiments, where deletion of the upaB gene in CFT073 significantly reduced early colonization of the bladder.
Keyword Biofilm
Autotransporter
Uropathogenic Escherichia-coli
Adhesin
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published ahead of print 19 September 2011

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Thu, 13 Oct 2011, 11:11:19 EST by Assoc Prof Mark Schembri on behalf of School of Chemistry & Molecular Biosciences