Sensitive quantification of somatic mutations using molecular inversion probes

Hirani, Rena, Connolly, Ashley R., Putral, Lisa, Dobrovic, Alexander and Trau, Matt (2011) Sensitive quantification of somatic mutations using molecular inversion probes. Analytical Chemistry, Articles ASAP 21: 8215-8221. doi:10.1021/ac2019409

Author Hirani, Rena
Connolly, Ashley R.
Putral, Lisa
Dobrovic, Alexander
Trau, Matt
Title Sensitive quantification of somatic mutations using molecular inversion probes
Journal name Analytical Chemistry   Check publisher's open access policy
ISSN 0003-2700
Publication date 2011-09-26
Sub-type Article (original research)
DOI 10.1021/ac2019409
Volume Articles ASAP
Issue 21
Start page 8215
End page 8221
Total pages 7
Place of publication Washington, DC, United States
Publisher American Chemical Society
Collection year 2012
Language eng
Abstract Somatic mutations in DNA can serve as cancer specific biomarkers and are increasingly being used to direct treatment. However, they can be difficult to detect in tissue biopsies because there is often only a minimal amount of sample and the mutations are often masked by the presence of wild type alleles from nontumor material in the sample. To facilitate the sensitive and specific analysis of DNA mutations in tissues, a multiplex assay capable of detecting nucleotide changes in less than 150 cells was developed. The assay extends the application of molecular inversion probes to enable sensitive discrimination and quantification of nucleotide mutations that are present in less than 0.1% of a cell population. The assay was characterized by detecting selected mutations in the KRAS gene, which has been implicated in up to 25% of all cancers. These mutations were detected in a single multiplex assay by incorporating the rapid flow cytometric readout of multiplexable DNA biosensors.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes ASAP articles are edited and published online ahead of print.

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Created: Thu, 13 Oct 2011, 09:39:38 EST by Dr Ashley Connolly on behalf of Aust Institute for Bioengineering & Nanotechnology