Crystallization and preliminary X-ray analysis of the chemokine-binding protein from orf virus (Poxviridae)

Counago, Rafael Miguez, Fleming, Stephen B., Mercer, Andrew A. and Krause, Kurt L. (2010) Crystallization and preliminary X-ray analysis of the chemokine-binding protein from orf virus (Poxviridae). Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 66 7: 819-823. doi:10.1107/S1744309110018166

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ255299_OA.pdf Full text (open access) application/pdf 294.57KB 0

Author Counago, Rafael Miguez
Fleming, Stephen B.
Mercer, Andrew A.
Krause, Kurt L.
Title Crystallization and preliminary X-ray analysis of the chemokine-binding protein from orf virus (Poxviridae)
Formatted title
Crystallization and preliminary X-ray analysis of the chemokine-binding protein from orf virus (Poxviridae)
Journal name Acta Crystallographica Section F: Structural Biology and Crystallization Communications   Check publisher's open access policy
ISSN 1744-3091
Publication date 2010-06
Sub-type Article (original research)
DOI 10.1107/S1744309110018166
Open Access Status File (Publisher version)
Volume 66
Issue 7
Start page 819
End page 823
Total pages 5
Place of publication Malden, MA, United States
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
The parapoxvirus orf virus (ORFV) encodes a chemokine-binding protein (CBP) that functions to downregulate the host's immune response at the site of infection by blocking the chemokine-induced recruitment of immune cells. In order to shed light on the structural determinants of CBP-chemokine binding, ORFV CBP was crystallized as part of an ongoing structure-function study on this protein. ORFV CBP crystals were obtained by the sitting-drop vapour-diffusion technique using ammonium citrate as a precipitant. The crystal quality was greatly improved through the addition of small-molecule additives to the crystallization mother liquor. ORFV CBP crystals diffracted X-rays to 2.50 Å resolution and belonged to the hexagonal space group P6122 or its enantiomorph P6522, with unit-cell parameters a = b = 75.62, c = 282.49 Å,α = 90, β = 90, γ = 120°.
Keyword Chemokine-binding proteins
Orf virus
Inflammation
Parapoxviruses
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: ERA 2012 Admin Only
School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 2 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 2 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 12 Oct 2011, 17:50:45 EST by System User on behalf of School of Chemistry & Molecular Biosciences