Accurate, reproducible measurement of acetone concentration in breath using selected ion flow tube-mass spectrometry

Dummer, Jack, Storer, Malina K., Hu, Wan-Ping, Swanney, Maureen P., Milne, Gordon J., Frampton, Christopher M., Scotter, Jenny M., Prisk, G. Kim and Epton, Michael J. (2010) Accurate, reproducible measurement of acetone concentration in breath using selected ion flow tube-mass spectrometry. Journal of Breath Research, 4 4: . doi:10.1088/1752-7155/4/4/046001


Author Dummer, Jack
Storer, Malina K.
Hu, Wan-Ping
Swanney, Maureen P.
Milne, Gordon J.
Frampton, Christopher M.
Scotter, Jenny M.
Prisk, G. Kim
Epton, Michael J.
Title Accurate, reproducible measurement of acetone concentration in breath using selected ion flow tube-mass spectrometry
Journal name Journal of Breath Research   Check publisher's open access policy
ISSN 1752-7155
1752-7163
Publication date 2010-12
Sub-type Article (original research)
DOI 10.1088/1752-7155/4/4/046001
Volume 4
Issue 4
Total pages 8
Language eng
Formatted abstract
Using selected ion flow tube-mass spectrometry (SIFT-MS) for on-line analysis, we aimed to define the optimal single-exhalation breathing manoeuvre from which a measure of expired acetone concentration could be obtained. Using known acetone concentrations in vitro, we determined the instrument's accuracy, inter-measurement variability and dynamic response time. Further, we determined the effects of expiratory flow and volume on acetone concentration in the breath of 12 volunteers and calculated intra-individual coefficients of variation (CVs). At acetone concentrations of 600–3000 ppb on 30 days over 2 months there was an instrument measurement bias of 8% that did not change over time, inter-day and intra-day CVs were 5.6% and 0.0%, respectively, and the 10–90% response time was 500 ± 50 ms (mean ± SE). Acetone concentrations at exhalation flows of 193 ± 18 (mean ± SD) and 313 ± 32 ml s−1 were 619 ± 1.83 (geometric mean ± logSD) and 618 ± 1.82 ppb in the fraction 70–85% by volume of exhaled vital capacity (V70−85%) and 636 ± 1.82 (geometric mean ± logSD) and 631 ± 1.83 ppb in V85−100%. A difference was observed between acetone concentrations in the V70−85% and V85−100% fractions (p < 0.01), but flow had no effect. Median intra-individual CVs were 1.6–2.6%. On-line SIFT-MS measurement of acetone concentration in a single exhalation requires control of exhaled volume but not flow, and yields low intra-individual CVs and is potentially useful in approximating blood glucose and monitoring metabolic stress.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Article # 046001

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Earth Sciences Publications
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Created: Wed, 12 Oct 2011, 17:44:05 EST by Wan-Ping Hu on behalf of School of Earth Sciences