Adiposity and adiponectin in dogs: investigation of causes of discrepant results between two studies

Verkest, K.R., Rose, F.J., Fleeman, L.M., Rand, J.S., Morton, J.M., Richards, A.A., Ishioka, K. and Whitehead, J.P. (2011) Adiposity and adiponectin in dogs: investigation of causes of discrepant results between two studies. Domestic Animal Endocrinology, 41 1: 35-41. doi:10.1016/j.domaniend.2011.03.004


Author Verkest, K.R.
Rose, F.J.
Fleeman, L.M.
Rand, J.S.
Morton, J.M.
Richards, A.A.
Ishioka, K.
Whitehead, J.P.
Title Adiposity and adiponectin in dogs: investigation of causes of discrepant results between two studies
Journal name Domestic Animal Endocrinology   Check publisher's open access policy
ISSN 0739-7240
Publication date 2011-07-01
Sub-type Article (original research)
DOI 10.1016/j.domaniend.2011.03.004
Volume 41
Issue 1
Start page 35
End page 41
Total pages 7
Place of publication United States
Publisher Elsevier Inc.
Collection year 2012
Language eng
Abstract Although one study showed lower adiponectin concentrations in obese dogs, other recent studies indicate that adiponectin might not be decreased in obese dogs, raising the possibility that the physiology of adiponectin is different in dogs than in humans. The aim of this study was to investigate possible causes of the discrepancy between the two largest studies to date that assessed the association between adiposity and adiponectin concentration in dogs, including the validity of the assay, laboratory error, and the effects of breed, sex, and neuter status on the relationship between adiposity and adiponectin concentrations. Adiponectin concentrations measured with a previously validated adiponectin ELISA were compared with those estimated by Western blotting analysis of reduced and denatured plasma samples. The possibility of laboratory error and the effect of EDTA anticoagulant and aprotinin were tested. Adiponectin concentration was measured by ELISA in 20 lean dogs (10 male and 10 female, 5 neutered in each sex). There was close correlation between adiponectin concentrations measured by ELISA and those estimated by Western blotting analysis (r = 0.90; P < 0.001). There was no substantial effect of EDTA, aprotinin, or laboratory error on the results. There was confounding by neuter status of the relationship between adiposity and adiponectin concentrations, but adiponectin concentrations were not significantly lower in male than in female lean dogs (females, 36 mg/L; males, 26 mg/L; P > 0.20) and were not significantly lower in intact than in neutered lean male dogs (intact, 28 mg/L; neutered, 23 mg/L; P = 0.49). We conclude that the adiponectin ELISA previously validated for use in dogs appears to be suitable for determination of canine adiponectin concentrations and that testosterone does not appear to have a strong effect on plasma adiponectin concentrations in dogs. Obesity might decrease adiponectin concentrations in intact but not in neutered dogs.
Keyword Adipokine
Testosterone
Adiponectin
Canine
Sex
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Erratum to Verkest et al. July 2011 articles [Domest. Anim. Endocrinol. 41 (2011) 24–34 and Domest. Anim. Endocrinol. 41 (2011) 35–41] Domestic Animal Endocrinology, Volume 41, Issue 3, October 2011, Page 162

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Veterinary Science Publications
 
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Created: Wed, 12 Oct 2011, 20:45:22 EST by Professor Jacquie Rand on behalf of School of Veterinary Science