Validation of analytical parameters of a competitive direct ELISA for aflatoxin B-1 in peanuts

Lee, N. A., Rachaputi, N. C., Wright, G. C., Krosch, S., Norman, K., Anderson, J., Ambarwati, S., Retnowati, I., Dharmaputra, O. S. and Kennedy, I. R. (2005) Validation of analytical parameters of a competitive direct ELISA for aflatoxin B-1 in peanuts. Food and Agricultural Immunology, 16 2: 149-163.


Author Lee, N. A.
Rachaputi, N. C.
Wright, G. C.
Krosch, S.
Norman, K.
Anderson, J.
Ambarwati, S.
Retnowati, I.
Dharmaputra, O. S.
Kennedy, I. R.
Title Validation of analytical parameters of a competitive direct ELISA for aflatoxin B-1 in peanuts
Formatted title Validation of analytical parameters of a competitive direct ELISA for aflatoxin B1 in peanuts
Journal name Food and Agricultural Immunology   Check publisher's open access policy
ISSN 0954-0105
1465-3443
Publication date 2005-06
Sub-type Article (original research)
DOI 10.1080/09540100500159179
Volume 16
Issue 2
Start page 149
End page 163
Total pages 15
Place of publication Colchester, United Kingdom
Publisher Taylor & Francis
Language eng
Formatted abstract Analytical validation of a competitive direct SUNQuik ELISA with a reference High Performance Liquid Chromatography (HPLC) method and other methods including a minicolumn method and the VICAM Aflatest® system for aflatoxin in peanuts was conducted. Both the ELISA and the VICAM Aflatest® system, using the same peanut extracts were analytically comparable with the HPLC method (R=0.998, p<0.000). The minicolumn method was also found to be acceptable as a low cost rapid semi-quantitative test. Despite the large variation in sampling, the correlation between the SUNQuik ELISA and HPLC using the different peanut sub-samples was considered acceptable over the range of 0–1200 µg kg−1 (R=0.938). No false negatives were found using the SUNQuik ELISA and false positives were either nil or negligible in all the studies conducted. The repeatability of the SUNQuik ELISA run on the same day was good with only±10% deviation. The reproducibility of the SUNQuik ELISA between days was also acceptable, but with a higher deviation. Applying the SUNQuik ELISA for aflatoxin surveys of peanuts in Indonesia proved that the method can deliver high quality, cost- and time-effective analysis with very little establishment capital and maintenance.
Keyword Aflatoxins
Human carcinogen
Elisa
Immunoassay
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
ERA 2012 Admin Only
 
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