Protein expression in the nucleus accumbens of rats exposed to developmental vitamin D deficiency

McGrath, John, Iwazaki, Takeshi, Eyles, Darryl, Burne, Thomas, Cui, Xiaoying, Ko, Pauline and Matsumoto, Izuru (2008) Protein expression in the nucleus accumbens of rats exposed to developmental vitamin D deficiency. PLoS One, 3 6: . doi:10.1371/journal.pone.0002383

Author McGrath, John
Iwazaki, Takeshi
Eyles, Darryl
Burne, Thomas
Cui, Xiaoying
Ko, Pauline
Matsumoto, Izuru
Title Protein expression in the nucleus accumbens of rats exposed to developmental vitamin D deficiency
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2008-06
Sub-type Article (original research)
DOI 10.1371/journal.pone.0002383
Open Access Status DOI
Volume 3
Issue 6
Total pages 7
Place of publication San Francisco, CA , United States
Publisher Public Library of Science
Language eng
Formatted abstract
Introduction: Developmental vitamin D (DVD) deficiency is a candidate risk factor for schizophrenia. Animal models have confirmed that DVD deficiency is associated with a range of altered genomic, proteomic, structural and behavioural outcomes in the rat. Because the nucleus accumbens has been implicated in neuropsychiatric disorders, in the current study we examined protein expression in this region in adult rats exposed to DVD deficiency

Methods: Female Sprague Dawley rats were maintained on a vitamin D deficient diet for 6 weeks, mated and allowed to give birth, after which a diet containing vitamin D was reintroduced. Male adult offspring (n = 8) were compared to control male (n = 8). 2-D gel electrophoresis-based proteomics and mass spectroscopy were used to investigate differential protein expression.

Results: There were 35 spots, mapped to 33 unique proteins, which were significantly different between the two groups. Of these, 22 were down-regulated and 13 up-regulated. The fold changes were uniformly small, with the largest FC being 21.67. Within the significantly different spots, three calcium binding proteins (calbindin1, calbindin2 and hippocalcin) were altered. Other proteins associated with DVD deficiency related to mitochondrial function, and the dynamin-like proteins. 

Conclusions: Developmental vitamin D deficiency was associated with subtle changes in protein expression in the nucleus accumbens. Disruptions in pathways related to calcium-binding proteins and mitochondrial function may underlie some of the behavioural features associated with animal models of developmental vitamin D deficiency 
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
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School of Medicine Publications
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