Antibody-Responses in the Lungs of Mice Following Oral Immunization with Salmonella-Typhimurium Aroa and Invasive Escherichia-Coli Strains Expressing the Filamentous Hemagglutinin of Bordetella-Pertussis

Guzman, Carlos A., Brownlie, Robert M., Kadurugamuwa, Jagath, Walker, Mark J. and Timmis, Kenneth N. (1991) Antibody-Responses in the Lungs of Mice Following Oral Immunization with Salmonella-Typhimurium Aroa and Invasive Escherichia-Coli Strains Expressing the Filamentous Hemagglutinin of Bordetella-Pertussis. Infection and Immunity, 59 12: 4391-4397.

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Author Guzman, Carlos A.
Brownlie, Robert M.
Kadurugamuwa, Jagath
Walker, Mark J.
Timmis, Kenneth N.
Title Antibody-Responses in the Lungs of Mice Following Oral Immunization with Salmonella-Typhimurium Aroa and Invasive Escherichia-Coli Strains Expressing the Filamentous Hemagglutinin of Bordetella-Pertussis
Journal name Infection and Immunity
ISSN 1098-5522
1070-6313
Publication date 1991-12
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 59
Issue 12
Start page 4391
End page 4397
Total pages 7
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract The filamentous hemagglutinin (FHA) of Bordetella pertussis was expressed in the attenuated aroA mutant of Salmonella typhimurium, SL3261, and in a strain of Escherichia coli harboring Shigella flexneri plasmid pWR110, which encodes bacterial invasiveness for epithelial cells. Expression of FHA in these strains did not interfere with their ability to invade Henle cells. Immunoglobulins A and G specific for FHA were detected in lung washes of mice following oral immunization with the live recombinant organisms; antibody levels were significantly higher than those in mice immunized with killed bacteria administered orally or intraperitoneally. Live oral vaccines carrying protective antigens of B. pertussis may be an important alternative to new-generation component vaccines against whooping cough.
Keyword Shigella-Flexneri
Infection Model
Hela-Cells
Vaccine
Plasmid
Antigen
Proteins
Iga
Purification
Efficacy
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
 
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