A second two-component regulatory system of Bordetella bronchiseptica required for bacterial resistance to oxidative stress, production of acid phosphatase, and in vivo persistence

Jungnitz, Heidrun, West, Nicholas P., Walker, Mark J., Chhatwal, Gursharan S. and Guzman, Carlos A. (1998) A second two-component regulatory system of Bordetella bronchiseptica required for bacterial resistance to oxidative stress, production of acid phosphatase, and in vivo persistence. Infection and Immunity, 66 10: 4640-4650.

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Author Jungnitz, Heidrun
West, Nicholas P.
Walker, Mark J.
Chhatwal, Gursharan S.
Guzman, Carlos A.
Title A second two-component regulatory system of Bordetella bronchiseptica required for bacterial resistance to oxidative stress, production of acid phosphatase, and in vivo persistence
Journal name Infection and Immunity
ISSN 1098-5522
1070-6313
Publication date 1998-10
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 66
Issue 10
Start page 4640
End page 4650
Total pages 11
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Formatted abstract
Random minitransposon mutagenesis was used to identify genes involved in the survival of Bordetella bronchiseptica within eukaryotic cells. One of the mutants which exhibited a reduced ability to survive intracellularly harbored a minitransposon insertion in a locus (ris) which displays a high degree of homology to two-component regulatory systems. This system exhibited less than 25% amino acid sequence homology to the only other two-component regulatory system described in Bordetella spp., the bvg locus. A risA′-′lacZtranslational fusion was constructed and integrated into the chromosome of B. bronchiseptica. Determination of β-galactosidase activity under different environmental conditions suggested that ris is regulated independently ofbvg and is optimally expressed at 37°C, in the absence of Mg2+, and when bacteria are in the intracellular niche. This novel regulatory locus, present in all Bordetella spp., is required for the expression of acid phosphatase by B. bronchiseptica. Although catalase and superoxide dismutase production were unaffected, the ris mutant was more sensitive to oxidative stress than the wild-type strain. Complementation of bvg-positive andbvg-negative ris mutants with the intact ris operon incorporated as a single copy into the chromosome resulted in the reestablishment of the ability of the bacterium to produce acid phosphatase and to resist oxidative stress. Mouse colonization studies demonstrated that the ris mutant is cleared by the host much earlier than the wild-type strain, suggesting that ris-regulated products play a significant role in natural infections. The identification of a second two-component system in B. bronchiseptica highlights the complexity of the regulatory network needed for organisms with a life cycle requiring adaptation to both the external environment and a mammalian host.
Keyword Gram-Negative Bacteria
Mouse Dendritic Cells
Salmonella-Typhimurium
Intracellular Survival
Signal-Transduction
Virulence Factors
Escherichia-Coli
Insertion Mutagenesis
Chromosomal Insertion
Superoxide-Dismutase
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Microscopy and Microanalysis Publications
 
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