Molecular detection and phylogenetic analysis of betanodavirus isolates from Australian finfish

Moody, N. J. G., Horwood, P. F., Reynolds, A., Mahony, T. J., Anderson, I. G. and Oakey, H. J. (2009) Molecular detection and phylogenetic analysis of betanodavirus isolates from Australian finfish. Diseases of Aquatic Organisms, 87 3: 151-160. doi:10.3354/dao02130

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Author Moody, N. J. G.
Horwood, P. F.
Reynolds, A.
Mahony, T. J.
Anderson, I. G.
Oakey, H. J.
Title Molecular detection and phylogenetic analysis of betanodavirus isolates from Australian finfish
Journal name Diseases of Aquatic Organisms   Check publisher's open access policy
ISSN 0177-5103
Publication date 2009-12-03
Sub-type Article (original research)
DOI 10.3354/dao02130
Open Access Status File (Publisher version)
Volume 87
Issue 3
Start page 151
End page 160
Total pages 10
Place of publication Oldendorf, Germany
Publisher Inter-Research
Language eng
Formatted abstract
In Australia, disease caused by betanodavirus has been reported in an increasing number of cultured finfish since the first report of mortalities in 1990. Partial coat protein gene sequences from the T2 or T4 regions of 8 betanodaviruses from barramundi Lates calcarifer, sleepy cod Oxyeleotris lineolata, striped trumpeter Latris lineata, barramundi cod Cromileptes altivelis, Australian bass Macquaria novemaculata and gold-spotted rockcod Epinephelus coioides from several Australian states were determined. Analysis of the 606 bp nucleotide sequences of the T2 region of 4 isolates demonstrated the close relationship with isolates from the red-spotted grouper nervous necrosis virus (RGNNV) genotype and the Cluster Ia subtype. Comparison of a smaller 289 bp sequence from the T4 region identified 2 distinct groupings of the Australian isolates within the RGNNV genotype. Isolates from barramundi from the Northern Territory, barramundi, sleepy cod, barramundi cod and gold-spotted rockcod from Queensland, and striped trumpeter from Tasmania shared a 96.2 to 99.7% nucleotide identity with each other. These isolates were most similar to the RGNNV genotype Cluster Ia. Isolates from Australian bass from New South Wales and from barramundi from South Australia shared a 98.6% sequence identity with each other. However, these isolates only shared an 85.8 to 87.9% identity with the other Australian isolates and representative RGNNV isolates. The closest nucleotide identity to sequences reported in the literature for the New South Wales and South Australian isolates was to an Australian barramundi isolate (Ba94Aus) from 1994. These 2 Australian isolates formed a new subtype within the RGNNV genotype, which is designated as Cluster Ic.
Keyword Betanodavirus
Nervous necrosis virus
Coat protein
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
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Citation counts: TR Web of Science Citation Count  Cited 11 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 10 Oct 2011, 10:53:23 EST by Dr Timothy Mahony on behalf of Qld Alliance for Agriculture and Food Innovation