Genes associated with fruit colour in Carica papaya: Identification through expressed sequence tags and colour complementation

Devitt, L. C., Dietzgen, R. G. and Holton, T. A. (2007). Genes associated with fruit colour in Carica papaya: Identification through expressed sequence tags and colour complementation. In: ISHS Acta Horticulturae 740: I International Symposium on Papaya. 1st International Symposium on Papaya, Genting Highlands, Malaysia, (133-140). 22-24 November 2005.

Author Devitt, L. C.
Dietzgen, R. G.
Holton, T. A.
Title of paper Genes associated with fruit colour in Carica papaya: Identification through expressed sequence tags and colour complementation
Formatted title Genes associated with fruit colour in Carica papaya: Identification through expressed sequence tags and colour complementation
Conference name 1st International Symposium on Papaya
Conference location Genting Highlands, Malaysia
Conference dates 22-24 November 2005
Convener A. R. Shukor
Proceedings title ISHS Acta Horticulturae 740: I International Symposium on Papaya   Check publisher's open access policy
Journal name Acta Horticulturae   Check publisher's open access policy
Place of Publication Leuven, Belgium
Publisher International Society for Horticultural Science
Publication Year 2007
Sub-type Fully published paper
ISBN 9789066052888
ISSN 0567-7572
Volume 740
Issue 740
Start page 133
End page 140
Total pages 8
Language eng
Formatted Abstract/Summary We have identified candidate genes that are differentially expressed during papaya fruit ripening in ‘Tainung’ (red-fleshed) and 1B (yellow-fleshed) hybrid varieties. Genes involved in carotenoid production were initially identified by data mining of expressed sequence tags (ESTs). Putative gene function was verified by colour complementation analysis using strains of Escherichia coli engineered to synthesise carotenoids or carotenoid precursors. In all, 1022 ESTs were searched with the BlastX algorithm against existing annotated plant gene databases, identifying seven putative carotenoid and aroma biosynthesis genes. Colour complementation identified papaya cDNA clones with significant homology to three carotenoid pathway genes. Gene expression analysis of these genes in two colour-contrasted papaya cultivars identified cultivar-specific differences in patterns of mRNA accumulation during fruit development. Differential expression of the two carotene desaturase encoding genes, phytoene desaturase and ζ-carotene desaturase and a gene encoding the carotene desaturase co-factor 4-hydroxy phenylpyruvate dioxygenase were identified and may be associated with colour phenotype differences in papaya.
Keyword Carotenoid biosynthesis
Est
mRNA accumulation
Gene discovery
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

 
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