The effect of stocking densities on reproductive performance in laboratory zebrafish (Danio rerio)

Castranova, Daniel, Lawton, Angela, Lawrence, Christian, Baumann, Diana P., Best, Jason, Coscolla, Jordi, Doherty, Amy, Ramos, Juan, Hakkesteeg, Jenna, Wang, Chongmin, Wilson, Carole, Malley, James and Weinstein, Brant M. (2011) The effect of stocking densities on reproductive performance in laboratory zebrafish (Danio rerio). Zebrafish, 8 3: 141-146. doi:10.1089/zeb.2011.0688


Author Castranova, Daniel
Lawton, Angela
Lawrence, Christian
Baumann, Diana P.
Best, Jason
Coscolla, Jordi
Doherty, Amy
Ramos, Juan
Hakkesteeg, Jenna
Wang, Chongmin
Wilson, Carole
Malley, James
Weinstein, Brant M.
Title The effect of stocking densities on reproductive performance in laboratory zebrafish (Danio rerio)
Formatted title
The effect of stocking densities on reproductive performance in laboratory zebrafish (Danio rerio)
Journal name Zebrafish   Check publisher's open access policy
ISSN 1545-8547
1557-8542
Publication date 2011-09-19
Sub-type Article (original research)
DOI 10.1089/zeb.2011.0688
Volume 8
Issue 3
Start page 141
End page 146
Total pages 6
Place of publication New Rochelle, NY, U.S.A.
Publisher Mary Ann Liebert, Publishers
Collection year 2012
Language eng
Formatted abstract
Despite the growing popularity of the zebrafish model system, the optimal husbandry conditions for this animal are not well defined. The aim of this study was to examine the effect of stocking density on reproductive performance in zebrafish. In this study, undertaken by eight different zebrafish facilities, clutches of at least 200 wild-type zebrafish embryos from a single pairwise mating were produced at each participating institution and subsequently reared according to “in-house protocols” until they were 14 weeks old. Fish were then randomly assigned into treatment groups with balanced sex ratios and densities of 3, 6, or 12 fish/L. After a 1-month acclimation period, fish were spawned in pair crosses every 2 weeks for 3 months, for a total of six spawning dates. The number of viable and nonviable embryos produced in each clutch were counted at 1 day postfertilization. Although there was a great deal of variability in clutch size and percent spawning success among laboratories, there were no significant differences in average clutch size, spawning success, or percent viable among the treatment densities. These data suggest that using stocking densities as high as 12 fish/L does not have a negative impact on performance, when measured by reproductive performance.
Keyword Salvelinus-alpinus
Crowding stress
Arctic charr
Growth
Juveniles
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Institute for Molecular Bioscience - Publications
 
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