Macromolecular NMR spectroscopy for the non-spectroscopist: Beyond macromolecular structure determination

Bieri, Michael, Kwan, Ann H., Mobli, Mehdi, King, Glenn F., Mackay, Joel P. and Gooley, Paul R. (2011) Macromolecular NMR spectroscopy for the non-spectroscopist: Beyond macromolecular structure determination. Febs Journal, 278 5: 704-715. doi:10.1111/j.1742-4658.2011.08005.x


Author Bieri, Michael
Kwan, Ann H.
Mobli, Mehdi
King, Glenn F.
Mackay, Joel P.
Gooley, Paul R.
Title Macromolecular NMR spectroscopy for the non-spectroscopist: Beyond macromolecular structure determination
Journal name Febs Journal   Check publisher's open access policy
ISSN 1742-464X
0945-5795
Publication date 2011-03
Sub-type Article (original research)
DOI 10.1111/j.1742-4658.2011.08005.x
Volume 278
Issue 5
Start page 704
End page 715
Total pages 12
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Collection year 2012
Language eng
Formatted abstract
A strength of NMR spectroscopy is its ability to monitor, on an atomic level, molecular changes and interactions. In this review, which is intended for non-spectroscopist, we describe major uses of NMR in protein science beyond solution structure determination. After first touching on how NMR can be used to quickly determine whether a mutation induces structural perturbations in a protein, we describe the unparalleled ability of NMR to monitor binding interactions over a wide range of affinities, molecular masses and solution conditions. We discuss the use of NMR to measure the dynamics of proteins at the atomic level and over a wide range of timescales. Finally, we outline new and expanding areas such as macromolecular structure determination in multicomponent systems, as well as in the solid state and in vivo.

Keyword Chemical shift mapping
NMR
NOE
Protein
Protein complex
Protein Dynamics
Protein folding
Protein interaction
Protein mutagenesis
Saturation difference
Q-Index Code CX
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
ERA White List Items
School of Chemistry and Molecular Biosciences
Institute for Molecular Bioscience - Publications
 
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Created: Fri, 07 Oct 2011, 13:51:28 EST by Professor Glenn King on behalf of School of Chemistry & Molecular Biosciences