An optimised transfection platform for the Epi-CHO transient expression system in serum-free media

Codamo, Joe, Munro, Trent P., Hughes, Benjamin S., Song, Michael and Gray, Peter P. (2012). An optimised transfection platform for the Epi-CHO transient expression system in serum-free media. In Nigel Jenkins, Niall Barron and Paula Alves (Ed.), Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009 (pp. 19-23) Dordrecht, Netherlands: Springer.

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Author Codamo, Joe
Munro, Trent P.
Hughes, Benjamin S.
Song, Michael
Gray, Peter P.
Title of chapter An optimised transfection platform for the Epi-CHO transient expression system in serum-free media
Formatted title An optimised transfection platform for the Epi-CHO transient expression system in serum-free media
Title of book Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009
Place of Publication Dordrecht, Netherlands
Publisher Springer
DOI 10.1007/978-94-007-0884-6_3
Publication year 2012
Sub-type Critical scholarly text
Year available 2011
Series ESACT proceedings
Editor Nigel Jenkins
Niall Barron
Paula Alves
ISBN 9789400708839; 9789400708846
Volume number 5
Chapter number 3
Start page 19
End page 23
Total pages 5
Total chapters 119
Collection year 2012
Language eng
Formatted Abstract/Summary Transient gene expression systems in mammalian cells continue to grow in popularity due to their capacity to produce significant amounts of recombinant protein in a rapid and scalable manner, without the lengthy time periods and resources required for stable cell line development. In this report, we demonstrate enhanced, high-level monoclonal antibody (mAb) titres of 136 mg/L with CHO cells using the episomal-based transient expression system, Epi-CHO. These high titres were achieved by implementing an optimised transfection protocol and through screening of a variety of chemically defined and serum-free media for their ability to support elevated and prolonged viable cell densities post-transfection, and in turn, improve recombinant protein yields. The Epi-CHO system allows for scalable and rapid production of CHO cell-derived recombinant proteins in serum-free conditions.
Q-Index Code B1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online: SpringerLink Date: Tuesday, September 13, 2011

 
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Created: Fri, 30 Sep 2011, 14:58:08 EST by Mr Benjamin Hughes on behalf of Aust Institute for Bioengineering & Nanotechnology