Separation and detection of bacteria using rupture event scanning

Cooper, Matthew A., Dultsev, Fedor N., Ostanin, Victor P. and Klenerman, David (2011) Separation and detection of bacteria using rupture event scanning. Analytica Chimica Acta, 702 2: 233-238. doi:10.1016/j.aca.2011.06.025


Author Cooper, Matthew A.
Dultsev, Fedor N.
Ostanin, Victor P.
Klenerman, David
Title Separation and detection of bacteria using rupture event scanning
Journal name Analytica Chimica Acta   Check publisher's open access policy
ISSN 0003-2670
1873-4324
Publication date 2011-09-30
Sub-type Article (original research)
DOI 10.1016/j.aca.2011.06.025
Volume 702
Issue 2
Start page 233
End page 238
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2012
Language eng
Abstract We have developed a sensitive and economical method to directly detect bacteria, based on the interaction between the bacteria and specific antibodies attached to an oscillating surface. By monotonously increasing the amplitude of oscillation of a quartz crystal microbalance (QCM) coated with the antibody, the QCM can be used to sensitively detect the acoustic noise produced when the interactions between the bacteria and the surface were broken. We term this process rupture event scanning (REVS). The method is quantitative over at least 6 orders of magnitude and can detect as few as 10 bacteria. We demonstrate here that this approach allows one to arrange separation of bacteria and follow the process completion on the basis of the acoustic signal. Detection is not significantly affected by non-specific binding of sample contaminants and thus can be achieved both in buffer and in serum.
Keyword Biosensor
Bond-rupture event
Separation
Quartz crystal microbalance
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Institute for Molecular Bioscience - Publications
 
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