Sox factors transcriptionally regulate ROBO4 gene expression in developing vasculature in zebrafish

Samant, Ganesh V., Schupp, Marcus O., Francois, Mathias, Moleri, Silvia, Kothinti, Rajendra K., Chun, Chang Zoon, Sinha, Indranil, Sellars, Suzanna, Leigh, Noah, Pramanik, Kallal, Horswill, Mark A., Remadevi, Indulehka, Li, Keguo, Wilkinson, George A., Tabatabai, Niloofar, Beltrame, M, Koopman, Peter A. and Ramchandran, Ramani (2011) Sox factors transcriptionally regulate ROBO4 gene expression in developing vasculature in zebrafish. Journal of Biological Chemistry, 286 35: 30740-30747. doi:10.1074/jbc.M111.220665

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Author Samant, Ganesh V.
Schupp, Marcus O.
Francois, Mathias
Moleri, Silvia
Kothinti, Rajendra K.
Chun, Chang Zoon
Sinha, Indranil
Sellars, Suzanna
Leigh, Noah
Pramanik, Kallal
Horswill, Mark A.
Remadevi, Indulehka
Li, Keguo
Wilkinson, George A.
Tabatabai, Niloofar
Beltrame, M
Koopman, Peter A.
Ramchandran, Ramani
Title Sox factors transcriptionally regulate ROBO4 gene expression in developing vasculature in zebrafish
Journal name Journal of Biological Chemistry   Check publisher's open access policy
ISSN 0021-9258
Publication date 2011-09
Sub-type Article (original research)
DOI 10.1074/jbc.M111.220665
Open Access Status File (Publisher version)
Volume 286
Issue 35
Start page 30740
End page 30747
Total pages 8
Place of publication Bethesda, MD, United States
Publisher American Society for Biochemistry and Molecular Biology
Collection year 2012
Language eng
Formatted abstract
Despite their importance as members of the Roundabout (Robo) family in the control of axonal and vascular patterning, the transcriptional regulation of these genes is poorly understood. In this study, we show that members of the Sry-related high mobility box (Sox) transcription factor family as being transcriptional regulators of roundabout4 (robo4), a Robo gene family member that participates in sprouting angiogenesis in vivo, in zebrafish. Double whole mount in situ hybridization analysis in zebrafish embryos revealed co-localization of the vascular relevant Sox factors sox7 or sox18 mRNA with robo4 transcripts in developing intersomitic vessels. A 3-kb human ROBO4 promoter element was able to drive reporter expression in zebrafish to recapitulate the endogenous temporal intersomitic vessel expression pattern of robo4. EMSA analysis confirmed binding of Sox18 to a canonical Sox binding site (from - 1170 bp to - 1176 bp) in the ROBO4 promoter (3 kb), and mutation analysis indicated that this site was partially responsible for ROBO4 promoter activity in ECs. A combination of gain- and loss-of-function analysis identified Sox7 and Sox18 co-regulation of robo4 but not fli1a transcripts in zebrafish. Finally, Sox-mediated robo4 transcriptional regulation is conserved across evolution. These studies imply Sox-mediated transcriptional regulation of Robo4 in the developing embryonic vasculature.
Keyword Redundant Roles
Lymphatic Vasculature
Neural Crest
In Vivo
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 9 times in Thomson Reuters Web of Science Article | Citations
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