Examination of the discrepancy between size estimates for ovalbumin from small-angle X-ray Scattering and other physicochemical measurements

Scott, David J., Patel, Trushar R., Besong, David M.T., Stetefeld, Jorg and Winzor, Donald J. (2011) Examination of the discrepancy between size estimates for ovalbumin from small-angle X-ray Scattering and other physicochemical measurements. Journal of Physical Chemistry B, 115 36: 10725-10729. doi:10.1021/jp2006149


Author Scott, David J.
Patel, Trushar R.
Besong, David M.T.
Stetefeld, Jorg
Winzor, Donald J.
Title Examination of the discrepancy between size estimates for ovalbumin from small-angle X-ray Scattering and other physicochemical measurements
Journal name Journal of Physical Chemistry B   Check publisher's open access policy
ISSN 1520-6106
Publication date 2011-09-01
Sub-type Article (original research)
DOI 10.1021/jp2006149
Volume 115
Issue 36
Start page 10725
End page 10729
Total pages 5
Place of publication Washington, DC United States
Publisher American Chemical Society
Collection year 2012
Language eng
Formatted abstract
Examination of the solution behavior of ovalbumin by small-angle X-ray scattering, dynamic light scattering, and analytical ultracentrifugation methods confirms its existence as a 44-kDa monomer in 20 mM phosphate, pH 7.0, thereby contradicting the discord introduced by published SAXS studies in favor of a dimeric state for this protein at neutral pH. Although the theoretical interpretation of SAXS measurements considers the consequences of thermodynamic nonideality arising from the repulsive interactions between molecules only if they give rise to a positive second virial coefficient, the fact that A 2 is negative for the present system does not account for the earlier findings. © 2011 American Chemical Society.
Keyword 2nd Virial-Coefficients
Flavus Urate Oxidase
Thermodynamic Nonideality
Sedimentation Equilibrium
Protein Interactions
Aqueous-Solutions
Ionic-Strength
Crystallization
Parameters
Molecules
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
ERA 2012 Admin Only
School of Chemistry and Molecular Biosciences
 
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