Changes in GFAP phosphorylation in the hypoxic/ischemic brain

Sullivan, S. M., Bjorkman, S. T., Miller, S. M. and Colditz, P. B. (2010). Changes in GFAP phosphorylation in the hypoxic/ischemic brain. In: 30th Annual Meeting of the Australian Neuroscience Society, Sydney, Australia, (). 1-3 February 2010.

Author Sullivan, S. M.
Bjorkman, S. T.
Miller, S. M.
Colditz, P. B.
Title of paper Changes in GFAP phosphorylation in the hypoxic/ischemic brain
Conference name 30th Annual Meeting of the Australian Neuroscience Society
Conference location Sydney, Australia
Conference dates 1-3 February 2010
Publication Year 2010
Sub-type Published abstract
Abstract/Summary Hypoxic/ischemic (H/I) brain damage is a leading cause of death and neurodevelopmental disability in neonates. Our previous research has suggested that astrocytes are important in determining the survival of neurons and thus the biology of astrocytes can influence overall outcomes after H/I brain damage. The astrocytic cytoskeletal protein glial fibrillary acidic protein (GFAP) can be phosphorylated at multiple sites by various enzymes. Phosphorylation shifts the equilibrium from the polymeric form to the less stable monomeric form of the protein. We have investigated whether the phosphorylation state of GFAP is altered after an H/I insult. Neonatal pigs (N=10) were anaesthetised and exposed to 4% oxygen for 30min, including 10min of ischemia. Control littermates (N=5) were exposed to anaesthesia, but not the H/I insult. Pigs were allowed to recover for 72hr, were euthanased and brain tissues removed. Slices from the left hemisphere were frozen for molecular/protein analyses and slices from the right hemisphere were fixed in paraformaldehyde for histology and immunohistochemistry. Polyclonal antibodies were generated in rabbits against the phosphorylated form of GFAP (pGFAP). Dot blots revealed that the antibodies specifically detected pGFAP and Western blots revealed a band (~50kDa), corresponding to the predicted molecular weight of the protein. Western blot analysis revealed a significant increase in pGFAP in the cortex of H/I animals (P<0.05) compared to controls. Immunohistochemical studies will determine whether increased pGFAP alters astrocyte morphology, which would influence neuronal survival after H/I insults. Future studies will examine whether therapies targeted at preventing or reversing GFAP phosphorylation offer an alternative pathway to neuroprotection in the neonatal H/I brain.
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Conference Paper
Collection: UQ Centre for Clinical Research Publications
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Created: Thu, 22 Sep 2011, 14:58:18 EST by Dr Susan Sullivan on behalf of UQ Centre for Clinical Research