Effects of exercise and antioxidant supplementation on endothelial gene expression

Matsumoto, Aya, Mason, Steven R., Flatscher-Bader, Traute, Ward, Leigh C., Marsh, Susan A., Wilce, Peter A., Fassett, Robert G., de Haan, udy B. and Coombes, Jeff S. (2012) Effects of exercise and antioxidant supplementation on endothelial gene expression. International Journal of Cardiology, 158 1: 59-65. doi:10.1016/j.ijcard.2010.12.104

Author Matsumoto, Aya
Mason, Steven R.
Flatscher-Bader, Traute
Ward, Leigh C.
Marsh, Susan A.
Wilce, Peter A.
Fassett, Robert G.
de Haan, udy B.
Coombes, Jeff S.
Title Effects of exercise and antioxidant supplementation on endothelial gene expression
Journal name International Journal of Cardiology   Check publisher's open access policy
ISSN 0167-5273
Publication date 2012
Year available 2011
Sub-type Article (original research)
DOI 10.1016/j.ijcard.2010.12.104
Volume 158
Issue 1
Start page 59
End page 65
Total pages 7
Place of publication Shannon, Co. Clare, Ireland
Publisher Elsevier Ireland
Collection year 2012
Language eng
Formatted abstract
The molecular mechanisms of exercise training induced cardiovascular protection are poorly understood. There is growing evidence that reactive oxygen species may be involved in a number of these adaptations and that antioxidants may be used to investigate this effect.

To determine the effects of exercise training and/or antioxidant supplementation on myocardial endothelium and vascular endothelium gene expression. Methods: Male Wistar rats were divided into four groups: i) control; ii) exercise trained (90 min of treadmill running 4d per week, 14 weeks); iii) antioxidant-supplemented (α-tocopherol 1000 IU kg- 1 diet and α-lipoic acid 1.6 g kg- 1 diet, mixed with rat chow) and iv) exercise trained and antioxidant-supplemented.

cDNA microarray analysis showed diverse expression changes in both left ventricular and coronary artery endothelial cells. In particular, RT-PCR analysis showed that a gene involved in cardiovascular disease progression, Ras homolog gene family member A, was down-regulated by exercise, and up-regulated by antioxidant supplementation in left ventricular endothelial cells. Furthermore, an important gene involved in inflammation, IL-6, was down-regulated by all treatments.


Exercise training and/or antioxidant supplementation affects cardiac endothelial cell gene expression, and their effects on genes such as ras homolog gene family member A and IL-6 provides insight into the molecular mechanisms of their influences on cardiovascular diseases.
Keyword Antioxidants
Endothelial cells
Gene expression
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online: 4 February 2011.

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Created: Thu, 22 Sep 2011, 14:15:58 EST by Dr Trudi Flatscher-bader on behalf of Queensland Brain Institute