Axonal regeneration proceeds through specific axonal fusion in transected C. elegans neurons

Neumann, Brent, Nguyen, Ken C. Q., Hall, David H., Ben-Yakar, Adela and Hilliard, Massimo A. (2011) Axonal regeneration proceeds through specific axonal fusion in transected C. elegans neurons. Developmental Dynamics, 240 6: 1365-1372. doi:10.1002/dvdy.22606


Author Neumann, Brent
Nguyen, Ken C. Q.
Hall, David H.
Ben-Yakar, Adela
Hilliard, Massimo A.
Title Axonal regeneration proceeds through specific axonal fusion in transected C. elegans neurons
Formatted title
Axonal regeneration proceeds through specific axonal fusion in transected C. elegans neurons
Journal name Developmental Dynamics   Check publisher's open access policy
ISSN 1058-8388
1097-0177
Publication date 2011-06
Sub-type Article (original research)
DOI 10.1002/dvdy.22606
Volume 240
Issue 6
Start page 1365
End page 1372
Total pages 8
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Collection year 2012
Language eng
Formatted abstract
Functional neuronal recovery following injury arises when severed axons reconnect with their targets. In Caenorhabditis elegans following laser-induced axotomy, the axon still attached to the cell body is able to regrow and reconnect with its separated distal fragment. Here we show that reconnection of separated axon fragments during regeneration of C. elegans mechanosensory neurons occurs through a mechanism of axonal fusion, which prevents Wallerian degeneration of the distal fragment. Through electron microscopy analysis and imaging with the photoconvertible fluorescent protein Kaede, we show that the fusion process re-establishes membrane continuity and repristinates anterograde and retrograde cytoplasmic diffusion. We also provide evidence that axonal fusion occurs with a remarkable level of accuracy, with the proximal re-growing axon recognizing its own separated distal fragment. Thus, efficient axonal regeneration can occur by selective reconnection and fusion of separated axonal fragments beyond an injury site, with restoration of the damaged neuronal tract.
Keyword Axonal fusion
Axonal regeneration
C. elegans
Axonal degeneration
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
Official 2012 Collection
 
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Citation counts: TR Web of Science Citation Count  Cited 27 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 20 Sep 2011, 15:50:54 EST by Dr Brent Neumann on behalf of Queensland Brain Institute