Rat insulin promoter 2-Cre recombinase mice bred onto a pure C57BL/6J background exhibit unaltered glucose tolerance

Fex, Malin, Wierup, Nils, Nitert, Marloes Dekker, Ristow, Michael and Mulder, Hindrik (2007) Rat insulin promoter 2-Cre recombinase mice bred onto a pure C57BL/6J background exhibit unaltered glucose tolerance. Journal of Endocrinology, 194 3: 551-555. doi:10.1677/JOE-07-0161


Author Fex, Malin
Wierup, Nils
Nitert, Marloes Dekker
Ristow, Michael
Mulder, Hindrik
Title Rat insulin promoter 2-Cre recombinase mice bred onto a pure C57BL/6J background exhibit unaltered glucose tolerance
Journal name Journal of Endocrinology   Check publisher's open access policy
ISSN 0022-0795
1479-6805
Publication date 2007-09
Sub-type Article (original research)
DOI 10.1677/JOE-07-0161
Volume 194
Issue 3
Start page 551
End page 555
Total pages 5
Place of publication Bristol, United Kingdom
Publisher BioScientifica
Language eng
Formatted abstract
β-Cell-specific gene targeting is a widely used tool when studying genes involved in β-cell function. For this purpose, several conditional β-cell knockouts have been generated using the rat insulin promoter 2-Cre recombinase (RIP2-Cre) mouse. However, it was recently observed that expression of Cre alone in β-cells may affect whole body glucose homeostasis. Therefore, we investigated glucose homeostasis, insulin secretion, and β-cell mass in our line of RIP2-Cre mice bred onto the C57BL/6J genetic background. We used 12- and 28-week-old female RIP2-Cre mice for analyses of insulin secretion in vitro, glucose homeostasis in vivo and β-cell mass. Our mouse line has been back crossed for 14 generationstoyieldanear100%pureC57BL/6J background. We found that fasting plasma glucose and insulin levels were similar in both genotypes. An i.v. glucose tolerance test revealed no differences in glucose clearance and insulin secretion between 12-week-old RIP2-Cre and WT mice. Moreover, insulin secretion in vitro in islets isolated from 28-week-old RIP2-Cre mice and controls was similar. In addition, β-cell mass was not different between the two genotypes at 28 weeks of age. In our experiments, we observed no differences in glucose tolerance, insulin secretion in vivo and in vitro, or in β-cell mass between the genotypes. As our RIP2-Cre mice are on a near 100% pure genetic background (C57BL/6J), we suggest that the perturbations in glucose homeostasis previously reported in RIP2-Cre mouse lines can be accounted for by differences in genetic background.
Keyword Tissue-Specific Knockout
Beta-Cell Mass
Cre Recombinase
Secretion
Receptor
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Erratum: Journal of Endocrinology December 1, 2007 Vol.195 Issue 3 p537.

Document type: Journal Article
Sub-type: Article (original research)
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