Apelin is a novel islet peptide

Ringstrom, Camilla, Nitert, Marloes Dekker, Bennet, Hedvig, Fex, Malin, Valet, Phillipe, Rehfeld, Jens F., Friis-Hansen, Lennart and Wierup, Nils (2010) Apelin is a novel islet peptide. Regulatory Peptides, 162 1-3: 44-51. doi:10.1016/j.regpep.2010.03.005

Author Ringstrom, Camilla
Nitert, Marloes Dekker
Bennet, Hedvig
Fex, Malin
Valet, Phillipe
Rehfeld, Jens F.
Friis-Hansen, Lennart
Wierup, Nils
Title Apelin is a novel islet peptide
Journal name Regulatory Peptides   Check publisher's open access policy
ISSN 0167-0115
Publication date 2010-06
Sub-type Article (original research)
DOI 10.1016/j.regpep.2010.03.005
Volume 162
Issue 1-3
Start page 44
End page 51
Total pages 8
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
Apelin, a recently discovered peptide with wide tissue distribution, regulates feeding behavior, improves glucose utilization, and inhibits insulin secretion. We examined whether apelin is expressed in human islets, as well as in normal and type 2 diabetic (T2D) animal islets. Further, we studied islet apelin regulation and the effect of apelin on insulin secretion. Apelin expression and regulation was examined in human and animal specimens using immunocytochemistry, in situ hybridization, and real-time PCR. Insulin secretion was studied in INS-1 (832/13) clonal beta cells. APJ-receptor expression was studied using real-time PCR. In human and murine islets apelin was predominantly expressed in beta cells and alpha cells; a subpopulation of the PP cells in human islets also harbored apelin. In porcine and feline islets apelin was mainly expressed in beta cells. APJ-receptor expression was detected in INS-1 (832/13) cells, and in human and mouse islets. A high dose (1 µM) of apelin-36 caused a moderate increase in glucose-stimulated insulin secretion (30%; p < 0.001), while lower concentrations (10–100 nM) of apelin robustly reduced insulin secretion by 50% (p < 0.001). Apelin was upregulated in beta cells of T2D db/db mice (47% vs. controls; p < 0.02) and GK-rats (74% vs. controls; p < 0.002), but human islet apelin expression was unaffected by glucose. On the other hand, human islet apelin expression was diminished after culture in glucocorticoids (16% vs. controls; p < 0.01). We conclude that apelin is a novel insulin-regulating islet peptide in humans and several laboratory animals. Islet apelin expression is negatively regulated by glucocorticoids, and upregulated in T2D animals. The presence of apelin receptors in islets suggests a role for apelin as a paracrine or autocrine messenger within the islets.
Keyword Apelin
APJ receptor
Insulin secretion
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: ERA 2012 Admin Only
School of Medicine Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 27 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 30 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 20 Sep 2011, 13:56:49 EST by System User on behalf of Royal Brisbane Clinical School