A transferable sucrose utilization approach for non-sucrose-utilizing Escherichia coli strains

Bruschi, Michele, Boyes, Simon J., Sugiarto, Haryadi, Nielsen, Lars K. and Vickers, Claudia E. (2012) A transferable sucrose utilization approach for non-sucrose-utilizing Escherichia coli strains. Biotechnology Advances, 30 5: 1001-1010.

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Author Bruschi, Michele
Boyes, Simon J.
Sugiarto, Haryadi
Nielsen, Lars K.
Vickers, Claudia E.
Title A transferable sucrose utilization approach for non-sucrose-utilizing Escherichia coli strains
Formatted title A transferable sucrose utilization approach for non-sucrose-utilizing Escherichia coli strains
Journal name Biotechnology Advances   Check publisher's open access policy
ISSN 0734-9750
1873-1899
Publication date 2012-09-01
Year available 2011
Sub-type Article (original research)
DOI 10.1016/j.biotechadv.2011.08.019
Volume 30
Issue 5
Start page 1001
End page 1010
Total pages 10
Place of publication New York, NY, U.S.A.
Publisher Elsevier
Collection year 2012
Language eng
Formatted abstract Sucrose has economic and environmental advantages over glucose as a feedstock for bioprocesses. E. coli is widely used in industry, but the majority of current industrial E. coli strains cannot utilize sucrose. Previous attempts to transfer sucrose catabolic capabilities into non-sucrose-utilizing strains have met with limited success due to low growth rates on sucrose and phenotypic instability of the engineered strains. To address these problems, we developed a transferrable sucrose utilization cassette which confers efficient sucrose catabolism when integrated onto the E. coli chromosome. The cassette was based on the csc genes from E. coli W, a strain which grows very quickly on sucrose. Both plasmid-borne expression and chromosomal integration of a repressor-less sucrose utilizing cassette were investigated in E. coli strains K-12, B and C. In contrast to previous studies, strains harboring chromosomal cassettes could grow at the same rate as they do on glucose. Interestingly, we also discovered that spontaneous chromosomal integration of the csc genes was required to allow efficient growth from plasmid-transformed strains. The ability to engineer industrial strains for efficient sucrose utilization will allow substitution of sucrose for glucose in industrial fermentations. This will encourage the use of sucrose as a carbon source and assist in transition of our petrochemical-based economy to a bio-based economy.
Keyword Transferrable sucrose utilization
Escherichia coli
Escherichia coli W
csc Genes
Chromosomal integration
Industrial bioprocess
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 1 September 2011.

 
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Created: Tue, 20 Sep 2011, 13:30:11 EST by Dr Claudia Vickers on behalf of Aust Institute for Bioengineering & Nanotechnology