In situ distribution and speciation of toxic copper, nickel, and zinc in hydrated roots of cowpea

Kopittke, Peter M., Menzies, Neal W., de Jonge, Martin D., McKenna, Brigid A., Donner, Erica, Webb, Richard I., Paterson, David J., Howard, Daryl L., Ryan, Chris G., Glover, Chris J., Schenkel, Kirk G. and Lombi, Enzo (2011) In situ distribution and speciation of toxic copper, nickel, and zinc in hydrated roots of cowpea. Plant Physiology, 156 2: 663-673. doi:10.1104/pp.111.173716

Author Kopittke, Peter M.
Menzies, Neal W.
de Jonge, Martin D.
McKenna, Brigid A.
Donner, Erica
Webb, Richard I.
Paterson, David J.
Howard, Daryl L.
Ryan, Chris G.
Glover, Chris J.
Schenkel, Kirk G.
Lombi, Enzo
Title In situ distribution and speciation of toxic copper, nickel, and zinc in hydrated roots of cowpea
Journal name Plant Physiology   Check publisher's open access policy
ISSN 0032-0889
Publication date 2011-06
Sub-type Article (original research)
DOI 10.1104/pp.111.173716
Volume 156
Issue 2
Start page 663
End page 673
Total pages 11
Place of publication Rockville, MD, U.S.A.
Publisher American Society of Plant Biologists
Collection year 2012
Language eng
Formatted abstract
The phytotoxicity of trace metals is of global concern due to contamination of the landscape by human activities. Using synchrotron-based x-ray fluorescence microscopy and x-ray absorption spectroscopy, the distribution and speciation of copper (Cu), nickel (Ni), and zinc (Zn) was examined in situ using hydrated roots of cowpea (Vigna unguiculata) exposed to 1.5 μM Cu, 5 μM Ni, or 40 μM Zn for 1 to 24 h. After 24 h of exposure, most Cu was bound to polygalacturonic acid of the rhizodermis and outer cortex, suggesting that binding of Cu to walls of cells in the rhizodermis possibly contributes to the toxic effects of Cu. When exposed to Zn, cortical concentrations remained comparatively low with much of the Zn accumulating in the meristematic region and moving into the stele; approximately 60% to 85% of the total Zn stored as Zn phytate within 3 h of exposure. While Ni concentrations were high in both the cortex and meristem, concentrations in the stele were comparatively low. To our knowledge, this is the first report of the in situ distribution and speciation of Cu, Ni, and Zn in hydrated (and fresh) plant tissues, providing valuable information on the potential mechanisms by which they are toxic.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Authors prepress title: "In situ distribution and speciation of toxic Cu, Ni, and Zn in hydrated roots of cowpea".

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Created: Tue, 20 Sep 2011, 12:26:01 EST by Dr Richard Webb on behalf of School of Agriculture and Food Sciences